REGIONAL DISTRIBUTION OF 11-BETA-HYDROXYSTEROID DEHYDROGENASE IN RAT-BRAIN
REGIONAL DISTRIBUTION OF 11-BETA-HYDROXYSTEROID DEHYDROGENASE IN RAT-BRAIN
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DOI：
10.1210/endo-128-4-1741
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发表时间：
1991-04-01
期刊：
影响因子：
4.8
通讯作者：
MONDER, C
中科院分区：
文献类型：
--
作者：
LAKSHMI, V;SAKAI, RR;MONDER, C
本文报道了大鼠脑内11-β-羟类固醇脱氢酶的活性和分布。 NADP可显著增加皮质酮氧化为11-脱氢皮质酮的反应; NADPH可增加逆反应。 皮质醇是一种不良底物。 从6日龄到成年，大鼠脑内均存在11-脱氢酶（11-DH）和11-氧化还原酶（11-OR）活性，且两者活性呈正相关。 最高的酶活性被发现在脑垂体，小脑，海马和皮质。 在嗅区、下丘脑、脑干、视前核和杏仁核中发现较低水平。 两种抗11-DH的抗血清，命名为56-125和56-126，与34 K组分反应，在Western印迹上质量对应于大鼠肝11-DH。 在与大鼠肝11-DH抗体56-125反应的所有脑区中的优势蛋白质种类为26 K mol wt。 抗血清56-126与26 K蛋白无交叉反应。 26 K组分不是34 K降解产物。 在大脑的每个区域，Western印迹分析表明，26 K带强度与酶活性成正比。 然而，26 K蛋白缺乏11-DH活性。 所有11-DH和11-OR活性均与34 K抗原相关。 数据表明11-DH在脑组织中的不均匀分布。 它们与11-DH可能赋予大脑控制活性糖皮质激素的细胞内水平的能力并以这种方式介导细胞内类固醇功能的概念一致。
The activity and distribution of 11-beta-hydroxysteroid dehydrogenase in rat brain is described. Oxidation of corticosterone to 11-dehydrocorticosterone was significantly increased by NADP; the reverse reaction was increased by NADPH. Cortisol was a poor substrate. Both 11-dehydrogenase (11-DH) and 11-oxoreductase (11-OR) activities were found in brains of rats from 6 days to adult, and 11-DH and 11-OR activities were positively correlated with each other. Highest enzyme activities were found in pituitary, cerebellum, hippocampus, and cortex. Lower levels were found in the olfactory region, hypothalamus, brain stem, preoptic nucleus, and amygdala. Two antisera to 11-DH, designated 56-125 and 56-126, reacted with a 34K component corresponding in mass to rat liver 11-DH on Western blots. The dominant species of protein in all brain regions reacting with rat liver 11-DH antibody 56-125, was at 26K mol wt. Antiserum 56-126 did not cross-react with the 26K protein. The 26K component was not a 34K degradation product. In each region of the brain, Western blot analysis showed that the 26K band intensity was directly proportional to enzyme activity. However, the 26K protein was devoid of 11-DH activity. All 11-DH and 11-OR activities were associated with the 34K antigen. The data demonstrate the nonuniform distribution of 11-DH in brain tissue. They are consistent with the notion that 11-DH may confer upon brain the ability to control intracellular levels of active glucocorticoids and in this way mediate steroid function within the cell.