Isolation of Bartonella henselae from domestic cats in Japan
Isolation of Bartonella henselae from domestic cats in Japan
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DOI：
10.1292/jvms.58.81
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发表时间：
1996-01-01
影响因子：
1.2
通讯作者：
Katsube, Y
中科院分区：
文献类型：
--
作者：
Maruyama, S;Nogami, S;Katsube, Y
1995年1月至3月期间，作者首次从日本33只家猫的3只（9.1%）的血液中分离出亨塞拉巴尔通体。这三只猫是一只1.5岁的雄性宠物猫，有尿潴留，以及一只6岁的雌性宠物猫，年龄不详，没有异常。取血于溶解-离心管（Wampole隔离管）中，培养于5%兔血心脏输注琼脂板上，35℃，5% CO2气氛。可见的微小粗糙菌落在孵化后14天发育。显微观察显示革兰氏阴性和多形性杆状体。从分离物中提取的DNA用两种引物进行PCR扩增，这两种引物都是柠檬酸立克次体合成酶基因的特异性引物。从扩增基因的TaqI和HhaI消化模式鉴定分离株为母鸡b。经证实，日本的猫的血液中含有B. henselae，并且猫作为该生物体的储存库发挥了重要作用。
During the period from January to March 1995, the authors first isolated Bartonella henselae from the blood of three (9.1%) of 33 domestic cats in Japan. The three cats were a 1.5-year male pet cat-old with urinary retention, and 6-year-old female pound and age-unknown female pet cats with no abnormalities. The blood was taken in a lysis-centrifugation tube (Wampole Isolator tube) and cultured on 5% rabbit-blood heart infusion agar plates at 35 degrees C in the 5% CO2 atmosphere. Visible tiny rough colonies developed 14 days after incubation. The isolates showed Gram-negative and pleomorphic rods in microscopic observation. The DNA extracted from the isolates was amplified by PCR using two primers, which were specific for the rikettsial citrate synthase gene. The isolates were identified as B. henselae from the patterns of digestion with TaqI and HhaI of the amplified gene. It was confirmed that cats in Japan harbored B. henselae in their blood, and that cats play a significant role as the reservoir of the organism.