ANALYSIS OF SOLUBLE HUMAN AND MOUSE INTERFERON-GAMMA RECEPTORS EXPRESSED IN EUKARYOTIC CELLS
ANALYSIS OF SOLUBLE HUMAN AND MOUSE INTERFERON-GAMMA RECEPTORS EXPRESSED IN EUKARYOTIC CELLS
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DOI：
10.1111/j.1432-1033.1992.tb17453.x
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发表时间：
1992-12-01
期刊：
影响因子：
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通讯作者：
GAROTTA, G
中科院分区：
文献类型：
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作者：
GENTZ, R;HAYES, A;GAROTTA, G
重组杆状病毒感染的昆虫细胞和哺乳动物中国仓鼠卵巢细胞产生了人和小鼠干扰素-γ受体的胞外区。在这两个系统中表达的受体被分泌到培养基中。它们的信号肽被切割掉，蛋白质在糖基化过程中表现出异质性，但不影响与干扰素γ或特定抗体的结合能力。可溶性小鼠受体的结合能力与细胞表面锚定受体的结合能力相似，而人的受体结合能力较低。所有的可溶性受体都能抑制干扰素与细胞受体的结合，并中和干扰素所发挥的抗病毒活性。因此，这些受体可用于结构/功能分析和体内研究。
The extracellular domains of the human and mouse interferon-gamma receptors were produced in insect Spodoptera frugiperda cells infected with recombinant baculoviruses and in mammalian Chinese-hamster-ovary cells. The receptors expressed in both systems are secreted into the culture medium. Their signal peptides are cleaved off and the proteins show heterogeneity in glycosylation which, however, does not affect the capacity to bind interferon gamma or specific antibodies. The soluble mouse receptors exhibit binding capacities similar to those of cell-surface-anchored receptors, whereas the human receptors exhibit a lower binding capacity. All soluble receptors inhibit the binding of interferon gamma to cellular receptors and neutralize the antiviral activity exerted by interferon gamma. These receptors could therefore be useful for structure/function analyses and in vivo studies.