In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas
In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas
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DOI：
10.1111/j.1365-2559.2011.03751.x
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发表时间：
2011-01-01
期刊：
影响因子：
6.4
通讯作者：
Nunes, Fabio D.
中科院分区：
文献类型：
--
作者：
Liborio, Tatiana N.;Acquafreda, Thais;Nunes, Fabio D.
目的：分析三个同源盒基因（HOXA7、PITX1和PRRX1）在口腔鳞状细胞癌（OSCC）中的表达及其与OSCC某些独特组织病理特征的关系，并与邻近非肿瘤上皮（NT）进行比较。方法和结果：生成地高辛标记的特异于各同源盒基因的核糖探针，并在冷冻切片上进行原位杂交。在NT样本中，HOXA7和PITX1转录本在所有上皮层中更常见，而PRRX1在基底层表达。在OSCC样本中，这三个基因的表达与所有组织学特征相关。然而，HOXA7和PITX1信号在小巢和离体细胞中更强烈，PRRX1信号在小巢和离体细胞中更强烈。结论：HOXA7、PIXT1和PRRX1同源盒基因在OSCC中有不同的表达模式，这取决于OSCC的组织学特征。
Aims:To analyse the expression of three homeobox genes (HOXA7, PITX1 and PRRX1) in oral squanous cell carcinomas (OSCC) and the relationship of such expression to certain distinct histopathological features of OSCC and in comparison to adjacent non-neoplastic epithelium (NT).Methods and results:Digoxigenin-labelled riboprobes that are specific for each homeobox gene were generated and in situ hybridization was carried out on frozen sections. In NT samples, HOXA7 and PITX1 transcripts were found more frequently in all epithelial layers, while PRRX1 was expressed in the basal layer. With OSCC samples, expression of the three genes was associated with all histological features. However, the HOXA7 and PITX1 signals were more intense in sheets and nests and PRRX1 in small nests and isolated cells.Conclusion:HOXA7, PIXT1 and PRRX1 homeobox genes have different patterns of expression in OSCC depending on its histological features.