Localization of nestin in amygdaloid kindled rat: An immunoelectron microscopic study
Localization of nestin in amygdaloid kindled rat: An immunoelectron microscopic study
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DOI：
10.1017/s0317167100003747
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发表时间：
2004-11-01
影响因子：
3
通讯作者：
Itano, T
中科院分区：
文献类型：
--
作者：
Nakagawa, T;Miyamoto, O;Itano, T
背景：巢蛋白是哺乳动物早期胚胎发育过程中表达的一类中间丝蛋白。出生后，巢蛋白及其mRNA下调并逐渐消失。近年来，在成人神经系统中检测到巢蛋白的表达，并提示该蛋白可能与神经发生有关，尽管其在神经发生中的作用机制尚不清楚。方法：利用光镜和电镜观察大鼠扁桃体点燃后中枢神经系统组织中巢蛋白的定位。结果：点燃动物主要在梨状皮质和鼻周皮质表达巢蛋白。光镜下，巢蛋白在星形胶质细胞、神经元和内皮细胞中表达。电镜显示内皮细胞、星形细胞血管末梢足、罕见的周细胞、神经元和少突胶质细胞中均有巢蛋白表达。结论：癫痫可引起CNS多种细胞（包括非神经细胞）广泛表达巢蛋白。
Background: Nestin is a class VI intermediate filament protein, expressed during early embryonic development in mammals. Postnatally, nestin and its mRNA are down-regulated and gradually disappear. Recently, nestin expression has been detected in the adult nervous system, and it has been suggested that this protein may be related to neurogenesis, although, its role in the mechanism of neurogenesis is not known. Methods: The present study examined the localization of nestin in CNS tissue of the amygdaloid kindled rat by light and electron microscopy. Results: Kindled animals showed nestin expression mainly in the piriform cortex and the perirhinal cortex. By light microscopy, nestin was shown to be expressed in astrocytes, neurons, and endothelial cells. Electron microscopy demonstrated nestin expression in endothelial cells, astrocytic perivascular end feet, the rare pericyte, neurons and oligodendrocytes. Conclusion: We conclude that epilepsy causes widespread nestin expression in many cell types in the CNS, including non-neural cells.