Antioxidative activity of apple skin or flesh extracts associated with fruit development on selected apple cultivars
Antioxidative activity of apple skin or flesh extracts associated with fruit development on selected apple cultivars
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DOI：
10.1016/s0304-4238(02)00127-9
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发表时间：
2002-12-06
影响因子：
4.3
通讯作者：
Ueda, J
中科院分区：
文献类型：
--
作者：
Kondo, S;Tsuda, K;Ueda, J
以3个不同苹果品种（Malussylvestris（L.）Mill.变种Artistica（Borkh.）Mansf.）;红皮的富士、黄皮的乌林和苹果酒。在果实发育过程中，三个品种果皮清除超氧阴离子（O-2（-））和DPPH自由基的IC 50值均较低。‘富士’和‘乌林’果肉中酶活性的IC_（50）值在开始时较低，但随着成熟期的增加而增加。相反，在整个果实发育过程中，'Redfield'果肉中活性的IC 50值保持较低。在三个品种的果皮和果肉中检测到绿原酸、根皮苷、（+）-儿茶素和（-）-表儿茶素等多酚类物质。总体而言，红场的果皮和果肉中的（+）-儿茶素和（-）-表儿茶素的浓度高于富士和大林。三个品种的果皮和果肉中总酚含量在鲜重基础上均随成熟期的延长而降低。而红田的总酚含量高于富士和大林。这些结果表明，苹果果皮和果肉中的自由基清除活性可能取决于多酚的浓度和组成。(C)2002 Elsevier Science B.V保留所有权利。
The antioxidative activity of skin or flesh extracts and the polyphenolic composition during the, fruit development were examined in three different cultivars of apple (Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.); 'Fuji' with red skin, 'Oorin' with yellow skin, and 'Redfield', a cider apple. The IC50 values of superoxide (O-2(-)) and 1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activities remained low throughout fruit development in the skin of the three cultivars. The IC50 values of the activities in the flesh of 'Fuji' and 'Oorin' were low at the beginning, but increased toward the ripening stage. In contrast, the IC50 values of the activities in the flesh of 'Redfield' remained low throughout fruit development. Chlorogenic acid, phloridzin, (+)-catechin, and (-)-epicatechin were detected as polyphenolics in the skin and flesh of the three cultivars. In general, the concentrations of (+)-catechin and (-)-epicatechin in the skin and flesh of 'Redfield' were higher than those in `Fuji' and 'Oorin'. Total phenolic concentrations on the fresh weight basis in the skin and flesh of three cultivars decreased towards the ripening stage. However, the total phenolic concentrations of 'Redfield' were higher than those of `Fuji' and 'Oorin'. These results demonstrate that the free-radical-scavenging activity in apple skin and flesh may depend on the concentration and composition of polyphenolics. (C) 2002 Elsevier Science B.V All rights reserved.