The analysis of circular dichroism spectra of natural DNAs using spectral components from synthetic DNAs
The analysis of circular dichroism spectra of natural DNAs using spectral components from synthetic DNAs
复制标题
使用合成 DNA 的光谱成分分析天然 DNA 的圆二色光谱
作者：
D. Gray;Frederick D. Hamilton;Marilyn R. Vaughan
我们测试了21种不同的合成DNA圆二色谱基组，并选择了一种用于天然DNA的光谱分析。该“标准”组由八种聚合物的光谱组成：聚[d（A-A-T）·d（A-T-T）]、聚[d（A-G-G）·d（C-C-T）]、聚[d（A-T）·d（A-T）]、聚[d（G-C）·d（G-C）]、聚[d（A-G）·d（C-T）]、聚[d（A-C）·d（G-T）]、聚[d（A-T-C）·d（G-A-T）]和聚[d（A-G-C）·d（G-C-T）]。该基组根据Gray和Tinoco的第一近邻聚合物程序应用，允许对六种天然复杂DNA和八种富含（A+T）的卫星DNA进行比以前更均匀准确的光谱分析，以获得碱基组成和第一近邻频率。我们发现poly[d（A）·d（T）]和/或poly[d（A-C-T-）·d（A-G-T）]的光谱通常不需要良好的分析结果，但我们在本文和后续论文中表明，这些光谱是某些卫星DNA最准确分析所必需的。
We have tested 21 different basis sets of synthetic DNA circular dichroism spectra and have slected one for use in spectral analyses of natural DNAs. This “standard” set consists of spectra of eight polymers: poly[d(A‐A‐T)·d(A‐T‐T)], poly[d(A‐G‐G)·d(C‐C‐T)], poly[d(A‐T)·d(A‐T)], poly[d(G‐C)·d(G‐C)], poly[d(A‐G)·d(C‐T)], poly[d(A‐C)·d(G‐T)], poly[d(A‐T‐C)·d(G‐A‐T)], and poly[d(A‐G‐C)·d(G‐C‐T)]. This basis set, applied according to the first‐neighbor polymer procedure of Gray and Tinoco, allows a more uniformly accurate spectral analysis of six natural complex DNAs and eight (A+T)‐rich satellite DNAs for base composition and first‐neighbor frequencies than was previously possible. We find that spectra of poly[d(A)·d(T)] and/or poly[d(A‐C‐T‐)·d(A‐G‐T)] are not generally required for good analysis results but we show in this and the following paper that these spectra are needed for the most accurate analyses of some satellite DNAs.