Antithetic population response to antibiotics in a polybacterial community
Antithetic population response to antibiotics in a polybacterial community
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DOI：
10.1126/sciadv.aaz5108
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发表时间：
2020-03-01
期刊：
影响因子：
13.6
通讯作者：
Garcia-Ojalvo, J.
中科院分区：
文献类型：
--
作者：
Galera-Laporta, L.;Garcia-Ojalvo, J.
关于抗生素对分离细菌种类的影响，我们知道得很多，但它们对多细菌群落的影响却知之甚少。在这里，我们研究了非耐药枯草芽孢杆菌和大肠杆菌混合菌群对中等浓度的β -内酰胺类抗生素氨苄西林的联合反应。我们发现，当两种微生物共存时，它们的种群对抗生素的反应与分离时相反：在单培养中，枯草芽孢杆菌对氨苄西林有耐受性，而大肠杆菌对氨苄西林敏感，在共培养中，大肠杆菌在抗生素存在下可以增殖，而枯草芽孢杆菌则不能。这种对立的行为是由一个数学模型预测的，该模型仅受两个物种孤立反应的约束。因此，我们的研究结果表明，混合细菌生态系统对抗生素的集体反应可能与单物种效力研究告诉我们的抗生素功效背道而驰。
Much is known about the effects of antibiotics on isolated bacterial species, but their influence on polybacterial communities is less understood. Here, we study the joint response of a mixed community of nonresistant Bacillus subtilis and Escherichia coli bacteria to moderate concentrations of the beta-lactam antibiotic ampicillin. We show that when the two organisms coexist, their population response to the antibiotic is opposite to that in isolation: Whereas in monoculture B. subtilis is tolerant and E. coli is sensitive to ampicillin, in coculture it is E. coli who can proliferate in the presence of the antibiotic, while B. subtilis cannot. This antithetic behavior is predicted by a mathematical model constrained only by the responses of the two species in isolation. Our results thus show that the collective response of mixed bacterial ecosystems to antibiotics can run counter to what single-species potency studies tell us about their efficacy.