Isolation and identification of a distinct side population cancer cells in the human epidermal squamous cancer cell line A431
Isolation and identification of a distinct side population cancer cells in the human epidermal squamous cancer cell line A431
复制标题
人表皮鳞状癌细胞系A431中独特侧群癌细胞的分离和鉴定
DOI：
10.1007/s00403-010-1100-1
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发表时间：
2011-04-01
影响因子：
3
通讯作者：
Zeng, Weihui
中科院分区：
文献类型：
--
作者：
Geng, Songmei;Wang, Qianqian;Zeng, Weihui
侧群（SP）细胞已被认为是多能癌症干细胞。为了研究表皮鳞癌细胞系A431中是否存在SP细胞，通过流式细胞术分选经Hoechst 33342染色的A431细胞。然后通过集落形成和细胞增殖测定分析SP细胞。进一步通过致瘤性和基因芯片分析比较SP细胞和非SP细胞的生物学差异。结果表明，A431细胞系中存在SP细胞，其增殖能力和集落形成能力均高于NSP细胞。在NOD/SCID小鼠中，SP细胞产生的肿瘤比NSP细胞产生的肿瘤大。mRNA芯片分析显示，5个癌标志基因表达上调，1个抑癌基因表达下调。这些结果表明，SP细胞在A431可能有助于自我更新，肿瘤转化和癌转移的人表皮鳞状细胞癌。
Side population (SP) cells have been suggested to be multipotent cancer stem cells. To address whether SP cells exist in epidermal squamous cancer cell line A431, A431 cells dyed with Hoechst 33342 were sorted through flow cytometry. The SP cells were then analyzed by colony-forming and cell proliferation assay. Further, tumorigenicity and microarray analysis were used to compare biological difference between SP and non-SP (NSP) cells. Our results showed that SP cells existed in the A431 cell line, showing higher proliferating and colony-forming ability than NSP cells. Tumors generated from SP cells were larger than those from the NSP cells in NOD/SCID mice. The mRNA microarray profiling revealed that five cancer marker gene expressions were up-regulated and one tumor suppressor gene expression was down-regulated. These findings suggest that SP cells in A431 could contribute to self-renewal, neoplastic transformation, and cancer metastasis of human epidermal squamous cell carcinoma.