Redundant roles of Sox17 and Sox18 in early cardiovascular development of mouse embryos
Redundant roles of Sox17 and Sox18 in early cardiovascular development of mouse embryos
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DOI：
10.1016/j.bbrc.2007.06.093
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发表时间：
2007-08-31
影响因子：
3.1
通讯作者：
Kanai, Yoshiakira
中科院分区：
文献类型：
--
作者：
Sakamoto, Youhei;Hara, Kenshiro;Kanai, Yoshiakira
Sox 7、-17和-18构成HMG盒转录因子基因的Sox亚组F（Sox F），它们都在小鼠发育中的血管内皮细胞中共表达。在这里，我们的特点是心血管表型的Sox 17/Sox 18双和Sox 17单无效胚胎在早期体节阶段。PECAM染色显示Sox，17个单空胚胎的异常心脏循环，主静脉扩大和前背主动脉形成的轻度缺陷。Sox 17/Sox 18双缺陷胚胎在前背主动脉和头/颈微血管形成方面表现出更严重的缺陷，在某些情况下，内皮细胞分化异常，内皮管融合缺陷。然而，后背主动脉和尿囊微血管在所有Sox 17/Sox 18双无效胚胎中均正常形成。前背主动脉和头颈部血管的异常与Sox 7弱表达部位相对应。这表明胚胎血管网前后轴上的三个SoxF成员沿着具有区域特异性的冗余活性。(c)2007年爱思唯尔公司All rights reserved.
Sox7, -17 and -18 constitute the Sox subgroup F (SoxF) of HMG box transcription factor genes, which all are co-expressed in developing vascular endothelial cells in mice. Here we characterized cardiovascular phenotypes of Sox17/Sox18-double and Sox 17-single null embryos during early-somite stages. Whole-mount PECAM staining demonstrated the aberrant heart looping, enlarged cardinal vein and mild defects in anterior dorsal aorta formation in Sox,17 single-null embryos. The Sox17/Sox18 double-null embryos showed more severe defects in formation of anterior dorsal aorta and head/cervical microvasculature, and in some cases, aberrant differentiation of endocardial cells and defective fusion of the endocardial tube. However, the posterior dorsal aorta and allantoic micro vasculature was properly formed in all of the Sox17/Sox18 double-null embryos. The anomalies in both anterior dorsal aorta and head/cervical vasculature corresponded with the weak Sox7 expression sites. This suggests the region-specific redundant activities of three SoxF members along the anteroposterior axis of embryonic vascular network. (c) 2007 Elsevier Inc. All rights reserved.