Comparative analysis of phenolic compound metabolism among tea plants in the section Thea of the genus Camellia
Comparative analysis of phenolic compound metabolism among tea plants in the section Thea of the genus Camellia
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茶属茶科茶树酚类化合物代谢比较分析
DOI：
10.1016/j.foodres.2020.109276
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发表时间：
2020-09-01
影响因子：
8.1
通讯作者：
Xia, Tao
中科院分区：
文献类型：
--
作者：
Liu, Yajun;Zhao, Guifu;Xia, Tao
野生茶树广泛分布于中国南方地区，属茶花科茶科，分不同种。从这些植物中生产出来的茶具有独特的风味，这与从茶叶品种中生产出来的茶不同。在这项研究中，我们对两种野生茶树（古井和四丘）和茶品种（舒茶早）的形态、系统发育关系和酚类化合物代谢进行了比较分析。四丘茶树和古井茶树具有相似的形态特征，在系统发育上可以归为一个簇，与广泛栽培的茶树（Camellia sinensis cv）完全分开。Shuchazao。代谢组学和转录组学分析表明，UGT84a22在古井叶片中的表达高于蜀茶早和四秋叶片，这可能是导致古井叶片中没糖酰奎宁酸积累较高的原因。F3’5’h启动子-765（-751）范围内的14 bp缺失可能导致四秋和古井茶树F3’5’h低表达水平，严重破坏了古井和四秋茶叶中三羟基黄酮类化合物的积累。古井茶的涩味强度高可能是由于原花青素和没食子酰奎宁酸积累较多。本研究的结果可以提高我们对茶花属茶科各进化群的代谢特征的认识。
Wild tea plants, which are classified into different species in the section Thea of the genus Camellia, are widely distributed in southern China. Tea produced from these plants has a unique flavor, which is different from that of tea produced from tea cultivars. In this study, we performed a comparative analysis of morphology, phylogenetic relationships, and phenolic compound metabolism between two wild tea plants (Gujing and Siqiu) and a tea cultivar (Shuchazao). Siqiu and Gujing tea plants had similar morphological traits and could be phylogenetically classified into a same cluster, which was entirely separate from the cluster containing widely cultivated cultivars such as Camellia sinensis cv. Shuchazao. Combined metabolomic and transcriptome analyses revealed that UGT84a22 was highly expressed in Gujing leaves compared with Shuchazao and Siqiu leaves, which may lead to the high accumulation of galloylquinic acid in Gujing leaves. A 14-bp deletion spanning the -765-(-751) range in the F3'5'H promoter potentially led to low F3'5'H expression levels in Siqiu and Gujing tea plants, which severely disrupted the accumulation of trihydroxy flavonoids in Gujing and Siqiu tea leaves. The high astringency intensity in Gujing tea could be due to the high accumulation of proanthocyanidins and galloyl-quinic acid. The results of the present study may improve our understanding of the metabolic characteristics of each evolutionary group of species or varieties in the section Thea of the genus Camellia.