Constitutive expression of three novel catfish CXC chemokines: Homeostatic chemokines in teleost fish
Constitutive expression of three novel catfish CXC chemokines: Homeostatic chemokines in teleost fish
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DOI：
10.1016/j.molimm.2004.12.012
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发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
Liu, ZJ
中科院分区：
文献类型：
--
作者：
Baoprasertkul, P;He, CB;Liu, ZJ
趋化因子最为人所知的是它们在白细胞趋化性中的重要作用，作为更大的炎症反应的一部分。在哺乳动物物种中的趋化因子的表达分析和功能表征往往忽略了这些蛋白质在稳态条件下的作用。最近的研究趋化因子的多样性硬骨鱼也集中在免疫相关的功能趋化细胞因子，如CXCL8和CXCL10。虽然基于疾病的趋化因子方法对于开发人类和动物感染的补救疗法至关重要，但它可能无法衡量趋化因子功能的整体复杂性。作为一个更大的努力，以评估在硬骨鱼的趋化因子多样性的保护的一部分，我们在这里报告的三个新的，组成型表达的CXC趋化因子从癍点叉尾鱼（Icalurus punctatus）的鉴定。系统发育分析表明，三个CXC趋化因子中的两个分别是哺乳动物CXCL 12和CXCL 14的直系同源物。虽然第三种CXC趋化因子还不能建立明确的同源性，但它与哺乳动物CXCL 2具有最高的氨基酸同一性。所有三种CXC趋化因子均显示在广泛的组织中表达，并且观察到CXCL12在发育期间的早期表达。这组新的鲶鱼CXC趋化因子的表达不诱导在挑战过程中感染的爱德华氏菌，病原体的鱼类肠道败血症的鲶鱼。与鲤鱼和斑马鱼中CXCL12的基因重复相反，Southern印迹分析表明，所有三种鲶鱼CXC趋化因子在鲶鱼基因组中均以单拷贝基因存在，这表明特定硬骨鱼中CXC趋化因子的基因重复是最近的进化事件。(c)2005爱思唯尔有限公司保留所有权利。
Chemokines are best known for their vital role in leukocyte chemotaxis, as part of the larger inflammatory response. Expression analysis and functional characterization of chemokines in mammalian species have often overlooked the role of these proteins under homeostatic conditions. Recent investigations of chemokine diversity in teleost fish have also centered on the immune-related functions of chemotactic cytokines, such as CXCL8 and CXCL10. While a disease-based approach to chemokines is essential to the development of remediative therapies for both human and animal infections, it may be a poor measure of the overall complexity of chemokine functions. As part of a larger effort to assess the conservation of chemokine diversity in teleost fish, we report here the identification of three novel, constitutively expressed CXC chemokines from channel catfish (Icalurus punctatus). Phylogenetic analyses indicated that two of the three CXC chemokines were orthologues for mammalian CXCL12 and CXCL 14, respectively. Whereas a clear orthology could not yet be established for the third CXC chemokine, it shared highest amino acid identity with mammalian CXCL2. All three CXC chemokines show expression in a wide range of tissues, and early expression during development was observed for CXCL12. The expression of this new set of catfish CXC chemokines was not induced during challenge by infection of Edwardsiella ictaluri, the causative agent of the fish pathogen enteric septicemia of catfish. In contrast to the gene duplication of CXCL12 in carp and zebrafish, Southern blot analysis indicated that all three catfish CXC chemokines exist as single copy genes in the catfish genome suggesting that gene duplication of CXC chemokines in specific teleost fish was a recent evolutionary event. (c) 2005 Elsevier Ltd. All rights reserved.