Maize Pollen Mutation by Low Energy Ion Beams
Maize Pollen Mutation by Low Energy Ion Beams
复制标题
低能离子束对玉米花粉的突变
DOI：
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发表时间：
2009-02
影响因子：
0.2
通讯作者：
Li Yongliang
中科院分区：
文献类型：
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作者：
Tang Jihua;Qin Guangyong;Huo Yuping;Li Yongliang
本研究建立了玉米花粉低能生活力测定方法。克服了离子注入花粉突变研究过程中遇到的几个挑战，包括真空泵吸过程中径流造成的花粉损失以及高花粉死亡率。结果表明，低能30 keV的1A-1014 N+ cm-2的半致死剂量处理玉米花粉效果最好。本研究以三个优良自交系为材料。在M_0和M_1群体中观察到一些突变体，如白色花药、红色种皮、种子致死突变体和白化病苗突变体。结果表明，低能离子束处理花粉是玉米突变育种的有效方法。关键词：玉米，花粉，低能离子束，诱变，辐射
A mensuration method to evaluate pollen viability with low energy in maize was established in this study. Several challenges experienced during ion implantation pollen mutation research were overcome, including pollen loss from run-off during vacuum pumping, and high pollen mortality rates. The data showed that a half lethal dose of 1A—1014 N+ cm-2 at low-energy 30 keV was most effective for treating maize pollen. Three elite inbred lines were treated in this study. Some mutants such as white anther color, red seed capsules, lethal seed and albino seedling mutants were observed in the M0 and M1 populations. The results demonstrated that the pollen treatment with low-energy ion beam was an effectual method in maize mutant breeding. Key Words: maize, pollen, low energy ion beams, mutation, radiation Â