Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7).
Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7).
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发表时间：
1993-09
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通讯作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
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作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
以前的生化研究已经确定，人的唾液中含有结构截然不同的高和低分子粘蛋白糖蛋白(分别为MG1和MG2)。在这项研究中，我们描述了重叠的编码mg2蛋白核心的cDNA克隆的分离和鉴定。DNA测序显示，该基因的翻译区为1131个核苷酸，编码377个氨基酸残基的蛋白质，分子质量为39 kDa。前20个N末端残基是非常疏水的，可能含有镁2先导肽。分泌蛋白的编码区域可分为三个不同的结构域：独特的5‘和3’翻译区，分别包含4个和1个潜在的N-糖基化位点，以及一个由23个氨基酸残基组成的6个近乎完美的串联重复序列的中心区，其中含有大量的Thr和Ser。没有发现与任何其他人或动物粘蛋白的序列同源性，也没有发现与任何其他蛋白质的显著同源性。Mg2基因全长约2.5kb，其表达具有物种特异性、组织特异性和细胞特异性。根据已克隆的粘蛋白基因命名为MUC1-MUC6，我们建议将该基因命名为MUC7。
Previous biochemical studies have determined that human saliva contains high and low molecular weight mucin glycoproteins (MG1 and MG2, respectively) that are structurally distinct. In this study, we describe the isolation and characterization of overlapping cDNA clones which code for the MG2 protein core. DNA sequencing revealed a translated region of 1131 nucleotides encoding a protein of 377 amino acid residues with a molecular mass of 39 kDa. The first 20 N-terminal residues were very hydrophobic and probably comprise the MG2 leader peptide. The region encoding the secreted protein can be divided into three distinct domains; unique 5'- and 3'-translated regions containing 4 and 1 potential N-glycosylation sites, respectively, and a central region of six almost perfect tandem repeats of 23 amino acid residues with a high number of Thr and Ser. No sequence homology with any other human or animal mucins, and no significant homology to any other proteins was found. MG2 mRNA is about 2.5 kilobases long, and its expression appears to be species-, tissue-, and cell-specific. We propose to name this gene MUC7 in accordance with the mucin genes cloned to date named MUC1-MUC6.