Plasmonic detection of a model analyte in serum by a gold nanorod sensor
Plasmonic detection of a model analyte in serum by a gold nanorod sensor
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DOI：
10.1021/ac0706527
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发表时间：
2007-07-15
影响因子：
7.4
通讯作者：
Chilkoti, Ashutosh
中科院分区：
文献类型：
--
作者：
Marinakos, Stella M.;Chen, Sihai;Chilkoti, Ashutosh
我们描述了一种基于金纳米棒的局域表面等离子体共振（LSPR）的无标记的芯片生物传感器的制造。将金纳米棒化学吸附到巯基硅烷改性的玻璃基底上，然后将生物素缀合到纳米棒上。通过固定化金纳米棒的UV-vis消光光谱中的LSPR峰的波长偏移来监测链霉亲和素与生物素的结合，所述波长偏移是由于链霉亲和素结合引起的金纳米棒表面处的局部折射率的变化。该传感器的检测限在PBS中为0.005 μ g/mL（94 pM），在血清中为1 μ g/mL（19 nM），动态范围为94 pM至0.19 μ M。基于纳米棒的传感器相对于我们先前从金纳米球（Nath，N.; Chilkoti，A. Anal. 2002，74，504-509; J. Chem. 2004，14，377-389; Anal. 2004，76，5370-5378）是纳米棒传感器的信号基于峰值波长偏移的测量提供的显著较低的检测限和内部自参考。
We describe the fabrication of a label-free, chip-based biosensor based on the localized surface plasmon resonance (LSPR) of gold nanorods. Gold nanorods were chemisorbed onto a mercaptosilane-modified glass substrate, followed by conjugation of biotin to the nanorods. Streptavidin binding to biotin was monitored by the wavelength shift of the LSPR peak in the UV-vis extinction spectrum of the immobilized gold nanorods due to the change in local refractive index at the gold nanorod surface induced by streptavidin binding. The limit of detection of the sensor is 0.005 mu g/mL (94 pM) in PBS and 1 mu g/mL (19 nM) in serum, and the dynamic range spans 94 pM to 0.19 mu M. The advantages of the nanorod-based sensor over an LSPR sensor that we had previously fabricated from gold nanospheres (Nath, N.; Chilkoti, A. Anal. Chem. 2002, 74, 504-509; J. Fluoresc. 2004, 14, 377-389; Anal. Chem. 2004, 76, 5370-5378) are the significantly lower detection limit and the internal self-reference that the signal of the nanorod sensor provides based on the measurement of peak wavelength shift.