Downregulation of HMGB1 protects against the development of acute lung injury after severe acute pancreatitis
Downregulation of HMGB1 protects against the development of acute lung injury after severe acute pancreatitis
复制标题
DOI：
10.1016/j.imbio.2013.04.013
复制
发表时间：
2013-10-01
期刊：
影响因子：
2.8
通讯作者：
Guo, Ren-Xuan
中科院分区：
文献类型：
--
作者：
Luan, Zheng-Gang;Zhang, Xiao-Juan;Guo, Ren-Xuan
目的：目的：探讨高迁移率族蛋白B1（HMGB 1）表达下调对重症急性胰腺炎（SAP）并发急性肺损伤（ALI）的影响及其对疾病严重程度的影响。胰管逆行注射5%牛磺胆酸钠诱发SAP。对照组仅接受假手术。在诱导SAP后收获肺和胰腺样品。检测肺组织中HMGB 1、基质金属蛋白酶-9（MMP-9）和细胞间粘附分子-1（ICAM-1）的蛋白水平。胰腺损伤的严重程度通过胰腺损伤的组织学评分、血清淀粉酶和胰腺含水量来确定。通过测定肺微血管通透性、肺髓过氧化物酶活性和丙二醛水平来评价肺损伤。结果发现，在pRNA-U6.1/Neo-HMGB 1处理的大鼠中，血清肿瘤坏死因子α（TNF-α）和白细胞介素-1 β与未处理的SAP或pRNA-U6.1/Neo.载体处理组大鼠（P
Objective: To examine the effect of downregulation of high mobility group box 1 (HMGB1) on severe acute pancreatitis (SAP) associated with acute lung injury (ALI), and its subsequent effect on disease severity.Methods: Wistar rats were given an IV injection of pRNA-U6.1/Neo-HMGB1, pRNA-U6.1/Neo-vector or saline before induction of SAP. Then, SAP was induced in rats by the retrograde injection of 5% sodium taurocholate into the pancreatic duct. The control group received only a sham operation. Lung and pancreas samples were harvested after induction of SAP. The protein levels of HMGB1, matrix metalloproteinase-9 (MMP-9) and intercellular adhesion molecule-1 (ICAM-1) in lung tissue were investigated. The severity of pancreatic injury was determined by a histological score of pancreatic injury, serum amylase, and pancreatic water content. The lung injury was evaluated by measurement of pulmonary microvascular permeability, lung myeloperoxidase activity and malondialdehyde levels.Results: The results found that in pRNA-U6.1/Neo-HMGB1 treated rats, serum tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) levels were decreased and the severity of pancreatic tissue injury was less compared with either untreated SAP or pRNA-U6.1/Neo-vector treated rats (P