Expression of focal adhesion kinase and α5 and β1 integrins in carcinomas and its clinical significance
Expression of focal adhesion kinase and α5 and β1 integrins in carcinomas and its clinical significance
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DOI：
10.3748/wjg.v8.i4.613
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发表时间：
2002-08-01
影响因子：
4.3
通讯作者：
Zha, XL
中科院分区：
文献类型：
--
作者：
Su, JM;Gu, L;Zha, XL
目的：检测粘着斑激酶(FAK)、整合素α5和β1亚基在不同类型癌组织中的表达规律，并探讨其与肿瘤类型、分级、淋巴结状态等临床病理参数的关系。方法：采用免疫组织化学方法检测75例胃癌、21例结直肠癌、16例肝细胞癌、20例宫颈癌和20例乳腺癌组织中FAK和整合素及其亚基的表达。FAK在低分化胃癌和结直肠癌中的表达增强。有淋巴结转移者FAK蛋白表达高于无转移者。此外，肿瘤的侵袭程度越深，FAK的表达越高。整合素α5和β1亚基在癌区的表达低于非癌区，在高分化癌组织中的表达高于低分化癌组织。整合素α5和β1亚基的表达与肿瘤的侵袭和转移无明显关系。FAK高表达(++或+)的癌组织中整合素α5和β1亚基在肿瘤及其未受影响的切缘也有较高的表达。结论：FAK是一个比整合素α5和β1亚基更好的肿瘤发生和发展的标志物，它不仅是一种转化相关酶，而且可能是一种进展相关的酶。
AIM: To detect the expression pattern of FAK (focal adhesion kinase) and integrin alpha5 and betai subunits in different kinds of cancerous tissues and to study their correlation with clinicopathological data including tumor type, grade and lymph node status. METHODS: Using an immunohistochemical technique, we examined the expression of FAK and integrin and subunits in cancerous and noncancerous tissues obtained from 75 patients with gastric carcinomas, 21 colorectal carcinomas, 16 hepatocellular carcinomas, 20 uterocervical carcinomas, and 20 breast carcinomas.RESULTS: The staining of FAK was stronger in cancerous than in noncancerous areas. Enhanced expression of FAK was detected in poor-differentiated carcinoma of the stomach and colorectum. Tumors with lymph node metastases had more FAK protein than those without metastases. In addition, the deeper the extent of tumor infiltration, the higher the FAK expression. The expression of integrin alpha5 and beta1 subunits was lower in cancerous areas than in noncancerous areas, but it was higher in well-differentiated cancerous tissues than in poor differentiated tissues. The relationship between the expression of integrin alpha5 and beta1 subunits and infiltration or metastasis was not significant. Cancerous tissues with stronger FAK expression (++ or +++) also had a higher expression of integrin alpha5 and beta1 subunits in the tumor and its unaffected margins.CONCLUSION: FAK is a better marker for carcinogenesis and the progression of cancer than integrin alpha5 or beta1 subunit, and it may be not only a transformation-linked enzyme but also a progression-linked enzyme.