Plasmodium falciparum apical membrane antigen 1 (PfAMA-1) is translocated within micronemes along subpellicular microtubules during merozoite development
Plasmodium falciparum apical membrane antigen 1 (PfAMA-1) is translocated within micronemes along subpellicular microtubules during merozoite development
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DOI：
10.1242/jcs.00665
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发表时间：
2003-09-15
影响因子：
4
通讯作者：
Mitchell, GH
中科院分区：
文献类型：
--
作者：
Bannister, LH;Hopkins, JM;Mitchell, GH
在裂殖阶段恶性疟原虫裂殖子的组装过程中，寄生虫合成并定位三组在红细胞入侵期间活跃的分泌囊泡（棒状体、微线体和致密颗粒）。每个裂殖子有多达40个微线体，形状像长颈瓶，长约160纳米，最宽直径为65纳米。它们的外表面上长有鬃毛状的细丝，每根粗细为 3-4 纳米，长为 25 纳米。微线体从靠近细胞核的单个高尔基样池沿着两个或三个膜下微管带转移到裂殖子顶端，在那里它们与棒状体尖端对接。高尔基池周围也形成致密颗粒，但其分布与微管无关。针对识别 83 kDa 和加工的 66 kDa 分子的重组 PfAMA-1 胞外域序列产生的三种多克隆抗体标记易位和成熟微线体的外围，但在裂殖体内裂殖子发育的任何阶段均不显着标记菱形。该结果证实PfAMA-1是一种微线体蛋白，并表明在微线体内它位于该细胞器的边界膜附近或插入该细胞器的边界膜中。
During the assembly of Plasmodium falciparum merozoites within the schizont stage, the parasite synthesizes and positions three sets of secretory vesicles (rhoptries, micronemes and dense granules) that are active during red cell invasion. There are up to 40 micronemes per merozoite, shaped like long-necked bottles, about 160 nm long and 65 nm at their widest diameter. On their external surfaces, they bear bristle-like filaments, each 3-4 nm thick and 25 nm long. Micronemes are translocated from a single Golgi-like cisterna near the nucleus along a band of two or three subpellicular microtubules to the merozoite apex, where they dock with the rhoptry tips. Dense granules are also formed around the periphery of the Golgi cisternae but their distribution is unrelated to microtubules. Three polyclonal antibodies raised against the recombinant PfAMA-1 ectodomain sequence recognizing both the 83 kDa and processed 66 kDa molecules label the peripheries of translocating and mature micronemes but do not label rhoptries significantly at any stage of merozoite development within schizonts. This result confirms that PfAMA-1 is a micronemal protein, and indicates that within the microneme it is located near or inserted into this organelle's boundary membrane.