Comparative investigation of the differentiation capability of bone-marrow- and adipose-derived mesenchymal stem cells by qualitative and quantitative analysis
Comparative investigation of the differentiation capability of bone-marrow- and adipose-derived mesenchymal stem cells by qualitative and quantitative analysis
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DOI：
10.1007/s00441-011-1306-3
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Ramesh, Thiyagarajan
中科院分区：
文献类型：
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作者：
Vishnubalaji, Radhakrishnan;Al-Nbaheen, May;Ramesh, Thiyagarajan
间充质干细胞（MSC）有望用于再生医学中的细胞治疗。迄今为止，MSC已经通过高度侵入性的程序从常规骨髓中获得。因此，MSC现在也从来源如脂肪组织、脐带血和脐带基质中分离，这是一个越来越受关注的主题。由于脂肪源性间充质干细胞（AD-MSCs）和骨髓源性间充质干细胞（BM-MSCs）的特性和分化潜力尚未被记录，我们通过定性和定量分析来评估和比较这两种类型的MSC的特性。两种细胞类型显示出相似的形态和表面蛋白表达，基质相关标志物阳性，造血和内皮标志物阴性。AD-MSCs的集落形成能力明显高于BM-MSCs。尽管如此，在两组MSC中观察到相似的成脂和成骨分化。细胞化学定性分析和钙矿化显示BM-MSCs的成骨分化水平高于AD-MSCs。相反，分化的脂肪细胞在AD-MSCs中的尼罗红油染色百分比高于BM-MSCs。定量实时聚合酶链反应显示两种细胞类型中成骨和成脂相关基因表达的相似模式。每一种MSC在功能分析中通过根据其微环境生态位在分化水平上表现出独特的特性来响应。因此，定量分析可能是一个有价值的手段描述干细胞多能性，除了定性调查。
Mesenchymal stem cells (MSCs) hold promise for cell-based therapy in regenerative medicine. To date, MSCs have been obtained from conventional bone marrow via a highly invasive procedure. Therefore, MSCs are now also isolated from sources such as adipose tissue, cord blood and cord stroma, a subject of growing interest. As the characterization and differentiation potential of adipose-derived MSCs (AD-MSCs) and bone-marrow-derived MSCs (BM-MSCs) have not been documented, we have evaluated and compared the characteristics of both MSC types by qualitative and quantitative analyses. Both cell types show similar morphology and surface protein expression, being positive for stromal-associated markers and negative for hematopoietic and endothelial markers. The colony-forming potential of AD-MSCs is distinctly higher than that of BM-MSCs. Nonetheless, similar adipogenic and osteogenic differentiation is observed in both groups of MSCs. Cytochemical qualitative analysis and calcium mineralization demonstrate higher levels toward osteogenic differentiation in BM-MSCs than in AD-MSCs. On the contrary, the percentage of Nile red oil staining for differentiated adipocytes is higher in AD-MSCs than in BM-MSCs. Quantitative real-time polymerase chain reaction shows similar patterns of osteogenic- and adipogenic-associated gene expression in both cell types. Each of the MSCs respond in functional analysis by exhibiting unique properties at the differentiation level according to their micro-environmental niche. Thus, quantitative analysis might be a valuable means of describing stem cell multipotency, in addition to qualitative investigation.