A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells
A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells
复制标题
DOI：
10.1016/j.yexcr.2008.12.024
复制
发表时间：
2009-04-15
影响因子：
3.7
通讯作者：
Sugiyama, Shin
中科院分区：
文献类型：
--
作者：
Furukawa, Kazuhiro;Ishida, Kazuya;Sugiyama, Shin
为了研究核纤层在体内的重组，在果蝇核纤层蛋白Dm（0）无效突变体脑细胞中人工表达果蝇A型和B型核纤层蛋白。外源性核纤层蛋白C（A型）和Dm（0）（B型）均在核周围形成亚层，并有效地逆转了NPC的异常聚集。核纤层蛋白C最初出现在NPC聚集的地方，随后沿着核周边延伸，并伴随着NPC规则分布的恢复。相反，核纤层蛋白Dm（0）在核纤层形成期间没有显示与成簇的NPC的缔合，并且NPC间距仅在完成闭合的核纤层蛋白Dm（0）层之后恢复。此外，当核纤层蛋白Dm（0）和C都表达时，它们不共融合，在不同区域中启动层形成。因此，A型和B型纤层蛋白在纤层组装过程中显示出不同的性质，A型在组织NPC分布中起主要作用。这种以前未知的复杂性，在组装的核层可能是复杂的核包膜功能的基础。(C)2008年由Elsevier Inc.出版
To investigate nuclear lamina re-assembly in vivo, Drosophila A-type and B-type lamins were artificially expressed in Drosophila lamin Dm(0) null mutant brain cells. Both exogenous lamin C (A-type) and Dm(0) (B-type) formed sub-layers at the nuclear periphery, and efficiently reverted the abnormal Clustering of the NPC. Lamin C initially appeared where NPCs were clustered, and subsequently extended along the nuclear periphery accompanied by the recovery of the regular distribution of NPCs. In contrast, lamin Dm(0) did not show association with the clustered NPCs during lamina formation and NPC spacing recovered only after completion of a closed lamin Dm(0) layer. Further, when lamin Dm(0) and C were both expressed, they did not co-polymerize, initiating layer formation in separate regions. Thus, A and B-type lamins reveal differing properties during lamina assembly, with A-type having the primary role in organizing NPC distribution. This previously unknown complexity in the assembly of the nuclear lamina could be the basis for intricate nuclear envelope functions. (C) 2008 Published by Elsevier Inc.