Corticosteroids and lymphoid cells in vitro. I. Hydrocortisone lysis of human, guinea pig, and mouse thymus cells.
Corticosteroids and lymphoid cells in vitro. I. Hydrocortisone lysis of human, guinea pig, and mouse thymus cells.
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体外皮质类固醇和淋巴细胞。
DOI：
10.5555/uri:pii:0022214371900862
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
W. Benner
中科院分区：
文献类型：
--
作者：
H. Claman;J. Moorhead;W. Benner
摘要 研究了氢化可的松在体外引起淋巴细胞裂解的能力。将小鼠、豚鼠和人胸腺细胞的悬浮液用 51 铬标记，并与不同浓度的氢化可的松琥珀酸钠一起孵育 6 至 24 小时。 51 Cr 释放量与 6 小时的细胞裂解相关，但与 24 小时的细胞裂解无关。 51 Cr 释放取决于时间和温度。小鼠胸腺细胞的裂解通过在10 -6 至10 -4 M范围内增加氢化可的松浓度而增加。在相同剂量范围内氢化可的松不增加豚鼠细胞的裂解。来自14名患者的人类胸腺细胞也未受到剂量为10 -8 至10 -3 M的氢化可的松的影响。来自另一名患者（最小的，年龄11个月）的胸腺细胞被较低浓度的氢化可的松裂解。这些数据强调了小鼠、豚鼠和人类淋巴细胞对皮质类固醇裂解敏感性的物种差异，证实了小鼠被称为“皮质类固醇敏感”，豚鼠和人类被称为“皮质类固醇抗性”。
Abstract The ability of hydrocortisone to cause lysis of lymphoid cells in vitro was studied. Suspensions of mouse, guinea pig, and human thymus cells were labelled with chromium-51 and incubated for 6 to 24 hours with varying concentrations of hydrocortisone sodium succinate. The amount of 51 Cr release correlated with cell lysis at 6 but not at 24 hours. 51 Cr release was time and temperature dependent. The lysis of mouse thymus cells was increased by increasing concentrations of hydrocortisone over a range of 10 −6 to 10 −4 M. Lysis of guinea pig cells was not increased by hydrocortisone in the same dose range. Human thymus cells from 14 patients were also not affected by hydrocortisone in doses of 10 −8 to 10 −3 M. Thymus cells from 1 other patient (the youngest, age 11 months) were lysed by lower concentrations of hydrocortisone. These data emphasize the species difference between mouse, guinea pig, and human lymphoid cells with regard to their sensitivity to lysis by corticosteroids, confirming the designations of mouse as "corticosteroid-sensitive" and guinea pig and man as "corticosteroid-resistant."