Intracellular recording from salamander olfactory receptor cells
Intracellular recording from salamander olfactory receptor cells
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蝾螈嗅觉受体细胞的细胞内记录
DOI：
10.1016/0006-8993(83)90488-2
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
P. Macleod
中科院分区：
文献类型：
--
作者：
D. Trotier;P. Macleod
从蝾螈嗅觉受体细胞获得细胞内记录。嗅觉神经纤维对逆向刺激的反应中出现的细胞内峰电位被认为是神经元刺穿的生理标准。平均静息电位为−56± 9 mV（平均值±S.D.; n= 70）。52个细胞呈现低于2个脉冲/s的自发峰电位。适当的嗅觉刺激通常诱发细胞内电位缓慢和分级下降（高达28 mV）。细胞的输入电阻在响应过程中显著降低。缓慢的电位变化引起重复放电。增加嗅觉刺激的强度增加了发射的瞬时频率（高达25 s-1），降低了尖峰幅度。尖峰在上升相呈现双光子，表明存在两个阶段的去极化。随着最强强度的刺激，脉冲活动停止在复极化阶段的细胞反应时，膜电位仍然明显去极化。
Intracellular recordings were obtained from salamander olfactory receptor cells. The occurrence of an intracellular spike in response to the antidromic stimulation of the olfactory fibers was considered as a physiological criterion of a neuronal impalement. The mean resting potential was−56±9mV (mean±S.D.;n= 70). Fifty-two cells presented a spontaneous spike activi lower than 2 impulses/s. Appropriate olfactory stimulation generally evoked a slow and graded decrease (up to 28 mV) of the intracellular potential. The input resistance of the cell decreased markedly during the response. The slow potential change induced a repetitive firing. Increasing the intensity of the olfactory stimulation increased the instantaneous frequency of firing (up to 25 s−1) are reduced the spike amplitude. The spike presented an inflexion in the rising phase indicating a two-stage depolarization. With the strongest intensities of stimulation the impulse activity was stopped during the repolarizing phase of the cell response when the membrane potential was still appreciably depolarized.