Cat and house dust mite allergen content is stable in frozen dust over time.
Cat and house dust mite allergen content is stable in frozen dust over time.
复制标题
随着时间的推移，冷冻灰尘中的猫和屋尘螨过敏原含量保持稳定。
DOI：
10.1021/es3052153
复制
发表时间：
2013
影响因子：
11.4
通讯作者：
C. Almqvist
中科院分区：
文献类型：
--
作者：
Anne;N. Andersson;C. Almqvist
背景
来自室内环境的灰尘包括动物过敏原、花粉、内毒素和其他可能加剧敏感个体症状的物质。在前瞻性队列研究中，通常从室内环境中收集灰尘，以评估过敏原暴露及其与健康结果的可能关系。通常，收集大量样本并冷冻保存，直到进一步分析，有时几年后。到目前为止，人们对储存过程中灰尘及其内容物的变化了解不够。
目标
在本研究中，我们的目的是分析过敏原含量超过30个月的时间内从床上收集的家庭，以模拟流行病学研究中常用的暴露评估的研究设计的冷冻灰尘。
方法
使用Duststream除尘器收集了37个来自家庭床垫的灰尘样品。每个粉尘样品被分成六等份。提取一个管（基线）并使用ELISA分析猫和屋尘螨（HDM）过敏原含量，所有其他管储存在-80 °C下直至进一步处理。大约每6个月（6、12、18和30个月），以相同的方式解冻、提取和分析一个试管中的灰尘。对数据进行对数转换，并使用线性回归进行分析。
结果
未观察到猫（p = 0.606）或HDM（p = 0.928）过敏原水平随时间降低或升高的趋势。与没有猫的家庭相比，有猫的家庭的床垫中猫过敏原的水平要高得多（p < 0.001）。
结论
重要的是，在设计昂贵和劳动密集型的过敏原暴露和健康结果的研究之前，评估粉尘中的过敏原稳定性，通常用于环境流行病学。尽管本研究表明，猫和HDM过敏原在-80 °C储存2.5年的灰尘中保持稳定，但需要对冷冻灰尘中的其他过敏原或物质进行分析，并评估较长储存时间的影响。
BACKGROUND
Dust from indoor environments consists of animal allergens, pollen, endotoxins, and other substances which may exacerbate symptoms in sensitive individuals. In prospective cohort studies, dust is often collected from indoor environments in order to assess allergen exposure and possible relationships to health outcomes. Typically, large numbers of samples are collected and kept frozen until further analysis, sometimes several years later. To date, there is insufficient knowledge about what happens to the dust and its contents during storage.
OBJECTIVES
In the present study, our aim was to analyze allergen content over a 30 month period frozen dust collected from beds in homes in order to simulate a study design of exposure assessment commonly used in epidemiological studies.
METHODS
Thirty-seven dust samples from mattresses in homes were collected using a Duststream dust collector. Each dust sample was subdivided into six aliquots. One tube (baseline) was extracted and analyzed for cat and house dust mite (HDM) allergen content using ELISA, all other tubes were stored at -80 °C until further handling. Approximately every six months (6, 12, 18, and 30 months), dust from one tube was thawed, extracted and analyzed the same way. Data was log-transformed and analyzed using linear regression.
RESULTS
No trend for decreasing or increasing cat (p = 0.606) or HDM (p = 0.928) allergen levels could be observed over time. Levels of cat allergen were considerably higher in mattresses from homes with cats compared to homes without cats (p < 0.001).
CONCLUSION
It is important to assess the allergen stability in dust before designing costly and labor-intensive studies of allergen exposure and health outcomes, commonly used in environmental epidemiology. Although the present study showed that cat and HDM allergens remained stable in dust stored at -80 °C during a 2.5 year period, analyses of other allergens or substances in frozen dust and evaluating the effect of longer storage times is desirable.