A selective group of dopaminergic neurons express Nurr1 in the adult mouse brain
A selective group of dopaminergic neurons express Nurr1 in the adult mouse brain
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DOI：
10.1016/s0006-8993(99)02149-6
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发表时间：
1999-12-18
期刊：
影响因子：
2.9
通讯作者：
Morales, M
中科院分区：
文献类型：
--
作者：
Bäckman, C;Perlmann, T;Morales, M
Nurr 1是核受体超家族的孤儿受体，广泛表达于中枢神经系统（CNS），包括多巴胺能神经元丰富的脑区。最近对Nurr 1无效突变小鼠的分析表明，Nurr 1对中脑多巴胺能神经元的发育和存活至关重要。然而，其他多巴胺能神经元群体似乎不受Nurr 1基因消融的影响。本研究的目的是调查Nurr 1 mRNA和酪氨酸羟化酶（TH）的免疫反应性在成年小鼠脑中的共存程度，以更好地表征Nurr 1对儿茶酚胺能神经元的选择性作用。结果表明，TH阳性神经元主要分布在黑质（SN; 96%）、腹侧被盖区（Ventral tegmental area，（VTA; Nurr 1在核团（95%）、红核后区（91%）、嗅球（85%）、中缝线状核（91%）和中央灰质（61%）表达。下丘脑室旁核和室周核的多巴胺能细胞仅显示少数Nurr 1/TH双标神经元，而弓状核和齿状回的TH阳性神经元不表达Nurr 1 mRNA。Nurr 1表达也被排除在脑干的肾上腺素能神经元之外。总之，Nurr 1转录本中没有发现所有的中枢神经系统儿茶酚胺能神经元。Nurr 1表达仅限于肾小球周围和中脑多巴胺能神经元。这些结果表明，在成年小鼠大脑中，Nurr 1可能参与嗅球和中脑的多巴胺能功能。(C)1999 Elsevier Science B. V.保留所有权利。
Nurr1, an orphan receptor of the nuclear receptor superfamily, is widely expressed in the central nervous system (CNS) including brain regions where dopaminergic neurons are abundant. Recent analyses of Nurr1 null mutant mice have shown that Nurr1 is essential for the development and survival of midbrain dopaminergic neurons. However, other dopaminergic neuronal populations do not seem to be affected by ablation of the Nurr1 gene. The purpose of the present study was to investigate the degree of co-existence of Nurr1 mRNA and tyrosine hydroxylase (TH) immunoreactivity in the brain of adult mice to better characterize the selective effects of Nurr1 on catecholaminergic neurons. Our results indicate that the majority of TH-immunoreactive neurons in the substantia nigra (SN; 96%), ventral tegmental area (VTA; 95%), retrorubral field (91%), olfactory bulb (85%), linear nucleus raphe (91%) and central grey (61%) express Nurr1, In contrast, dopaminergic cells of the paraventricular and periventricular hypothalamic nucleus showed only a few Nurr1/TH double labeled neurons, while TH-immunoreactive neurons in the arcuate nucleus and zona incerta did not express Nurr1 mRNA. Nurr1 expression was also excluded from (nor)adrenergic neurons of the brainstem. In conclusion, Nurr1 transcripts were not found in all CNS catecholaminergic neurons. Nurr1 expression was confined to periglomerular and midbrain dopaminergic neurons. These results suggest that within the adult mouse brain, Nurr1 may participate in dopaminergic functions of the olfactory bulb and midbrain. (C) 1999 Elsevier Science B.V. All rights reserved.