Physiological function of regenerating endothelium.
Physiological function of regenerating endothelium.
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发表时间：
1976-02
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影响因子：
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通讯作者：
A. Khodadoust;K. Green
中科院分区：
文献类型：
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作者：
A. Khodadoust;K. Green
在兔角膜上形成一个限定区域，直径为4 mm的冷冻损伤。在内皮损伤后的肿胀期和恢复期，在从利姆布斯到角膜中心的四个距离处测量角膜厚度。快速初始溶胀之后是超过24小时的稳定最大厚度的时期。中央肿胀多于外周肿胀。伤后8天，周边部角膜厚度恢复正常，10 ~ 12天后中央部角膜厚度恢复正常。角膜内皮组织学检查显示，细胞早期迁移到裸露区发生在伤后6小时。到第2天，大部分裸露区域被内皮细胞覆盖，尽管细胞较大且不规则。在接下来的五天内，正常细胞的数量增加，不规则细胞的数量减少，直到冷冻后两周，所有细胞都具有正常的外观。生理内皮功能的恢复滞后于组织学恢复约4 - 5天，表明正常内皮通透性的恢复可能与细胞连接的状态有关，而不是细胞本身覆盖后表面。
A defined area, 4 mm. diameter, cryothermal injury was created on a rabbit cornea. Corneal thickness was measured at four distances from the limbus to the center of the cornea during the swelling phase after endothelial damage, and during the recovery period. Rapid initial swelling was followed by a period of stable maximum thickness over 24 hours. More swelling occurred centrally than peripherally. Eight days after injury the peripheral cornea regained normal thickness, and the central portion was normal thickness after 10 to 12 days. Histological examination of corneal endothelium showed that early migration of cells into the denuded area occurred 6 hours after injury. By 2 days, most of the denuded area was covered by endothelial cells, although the cells were large and irregular. The number of normal cells increased, and of irregular cells decreased, over the next five days, until two weeks after freezing all cells had a normal appearance. The recovery of physiologic endothelial function lags behind the histologic recovery by about four to five days, indicating that recovery of the normal endothelial permeability is possibly related to the status of the cellular junctions rather than covering of the posterior surface by cells per se.