Expression of dectin-1 during fungus infection in human corneal epithelial cells
Expression of dectin-1 during fungus infection in human corneal epithelial cells
复制标题
人角膜上皮细胞真菌感染过程中dectin-1的表达。
DOI：
10.3980/j.issn.2222-3959.2014.01.06
复制
发表时间：
2014-02-18
影响因子：
1.4
通讯作者：
Qiu, Sheng
中科院分区：
文献类型：
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作者：
Li, Cui;Zhao, Gui-Qiu;Qiu, Sheng
目的：探讨树突状细胞相关C型凝集素-1 （dectin-1）在真菌感染的人角膜上皮（HCE）细胞中的表达。方法：将20例健康供体角膜作为A组，20例真菌性角膜炎（FK）患者（20只眼）作为b组。采用实时荧光定量pcr和免疫组化技术检测两组角膜上皮中检测蛋白-1的表达。用烟曲霉（aspergillus fumigatus， AF）抗原培养HCE细胞，分别在刺激0、4、8和24h时采用real - time qPCR检测dectin-1 mRNA的表达。免疫细胞化学分别在0和24h检测Dectin-1蛋白。结果：Dectin-1在正常人和FK患者角膜上皮中均有表达。体外细胞实验显示，AF抗原刺激后4h HCE细胞中dectin -1 mRNA表达开始升高，24h刺激后dectin -1蛋白表达升高。结论：Dectin -1在正常人角膜上皮中表达。AF抗原刺激可提高体外HCE细胞中dectin -1的表达。
AIM: To evaluate the expression of dendritic cell associated C type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus.METHODS: A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real time qPCR and immunohistochemistry were applied to detect dectin -1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus (AF) antigens in lent The expression of dectin-1 mRNA was measured by real time qPCR at the stimulation of 0, 4, 8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately.RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin -1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h, and dectin -1 protein expression increased after stimulation at 24h.CONCLUSION: Dectin -1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin -1 in HCE cells in vitro.