Longitudinal modulation of immune system cytokine profile during pregnancy.
Longitudinal modulation of immune system cytokine profile during pregnancy.
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DOI：
10.1016/j.cyto.2010.11.005
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发表时间：
2011-02
期刊：
影响因子：
3.8
通讯作者：
Culhane JF
中科院分区：
文献类型：
--
作者：
Denney JM;Nelson EL;Wadhwa PD;Waters TP;Mathew L;Chung EK;Goldenberg RL;Culhane JF
通过细胞因子试验表征人类妊娠三个时间点的免疫调节。这是一项前瞻性的纵向研究，研究单胎妊娠期间细胞因子表达的广泛影响，导致无并发症的足月活产。在妊娠8-14周、18-22周和28-32周采集外周血。用PHA或LPS刺激全血，并与未刺激的对照组进行比较，在上清液中测定6种细胞因子IFN-γ、IL-4、TNF-α、IL-1β、IL-6和IL-10。通过Luminex-100 MAP®处理样品。我们使用广义线性模型（GLM）来评估细胞因子的轨迹。完整的数据获得了45个简单的怀孕。总体而言，外周血WBC表现出抑制的细胞因子应答。然而，在怀孕过程中，我们发现增强的反调节细胞因子表达（例如，增加的IL-10）。随着无并发症妊娠的进展，促炎细胞因子的总体减少和反调节细胞因子的增加支持了维持可行妊娠的免疫调节的不断发展的概念。
To characterize immune modulation as expressed by cytokine assays at three time-points in human pregnancy. This is a prospective, longitudinal study of a broad panel of cytokine expression during singleton pregnancies resulting in an uncomplicated, full-term, live births. Peripheral blood was obtained at 8–14, 18–22, and 28–32 weeks gestation. Six cytokines—IFN-γ, IL-4, TNF-α, IL-1β, IL-6, and IL-10—were measured in supernatants obtained from whole blood stimulations with PHA or LPS and were compared to unstimulated controls. Samples were processed by Luminex-100 MAP®. We used Generalized Linear Models (GLM) to evaluate cytokine trajectories. Complete data were obtained for forty-five uncomplicated pregnancies. Overall, peripheral blood WBC’s demonstrated dampened cytokine responses. However, over the course of pregnancy, we found enhanced counter-regulatory cytokine expression (e.g., shown by increased IL-10). The overall decrease in pro-inflammatory cytokines and increase in counter-regulatory cytokines as uncomplicated pregnancy progresses supports the evolving concepts of immunoregulation for the maintenance of a viable pregnancy.