Heritability and identification of QTLs and underlying candidate genes associated with the architecture of the grapevine cluster (Vitis vinifera L.)
Heritability and identification of QTLs and underlying candidate genes associated with the architecture of the grapevine cluster (Vitis vinifera L.)
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DOI：
10.1007/s00122-014-2286-y
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发表时间：
2014-05-01
影响因子：
5.4
通讯作者：
Hinrichsen, P.
中科院分区：
文献类型：
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作者：
Correa, J.;Mamani, M.;Hinrichsen, P.
我们已经确定了19个轴结构的QTL，这是葡萄藤生产的一个关键和复杂的性状。在1,173个QTL中，有50个基因有待进一步研究。摘要在鲜食葡萄产业中，葡萄轴结构具有重要的经济和管理意义。因此，了解这种性状的遗传是其育种的关键。这项工作的目的是确定与集群结构相关的性状的遗传决定因素。在施用和不施用赤霉酸（GA（3））的情况下，在三个季节期间对'红宝石无核' × '苏丹尼娜'杂交（F-1：n = 137）的八个性状进行表征。几个性状的基因型效应和基因型× GA（3）互作显著。轴长、侧肩长和沿中轴沿着的节数是最显著的性状。这些性状的平均遗传力接近71%，其中rl在不同季节的遗传力为76%。数量性状位点（QTL）分析结果表明，连锁群5（LG（5））和LG（18）对这些性状的QTL数目最多。根据所解释的方差，在LG（9）上发现了主效QTL（对应于rl）。这些QTL主要由父本加性效应支持，并揭示了可能的多效性效应。基于葡萄藤参考基因组，我们确定了1,173个位于这些QTL置信区间内的基因。该列表的891个注释基因中的50个被选择用于它们的进一步表征，因为它们可能参与轴结构。所检测到的QTL表明这些性状及其对GA（3）的反应具有明确的遗传基础。由于解释的总方差的百分比，他们是很好的候选人，参与集群架构的遗传决定。
Key message We have identified 19 QTLs for rachis architecture, a key and complex trait for grapevine production. Fifty out of 1,173 genes underlying these QTLs are candidates to be further explored.Abstract In the table grape industry, the rachis architecture has economic and management implications. Therefore, understanding the genetics of this trait is key for its breeding. The aim of this work was to identify genetic determinants of traits associated with the cluster architecture. Characterisations of eight traits was performed on a 'Ruby Seedless' x 'Sultanina' crossing (F-1: n = 137) during three seasons, with and without gibberellic acid (GA(3)) applications. The genotypic effects and the genotype x GA(3) interactions were significant for several traits. Rachis length (rl), lateral shoulder length and node number along the central axis were the most prominent traits. On average, the heritability of these traits was similar to 71 %, with heritability of rl being 76 % as estimated under different seasons. Quantitative trait loci (QTLs) analyses showed that linkage group 5 (LG(5)) and LG(18) harboured the largest number of QTLs for these traits. According to the variance explained, the main QTL (corresponding to rl) was found on LG(9). These QTLs were supported mainly by a paternal additive effect and revealed possible pleiotropic effects. Based on the grapevine reference genome, we identified 1,173 genes located under these QTL confidence intervals. Fifty of the 891 annotated genes of this list were selected for their further characterisation because of their possible participation in the rachis architecture. In conclusion, the QTLs detected indicate that these traits and their GA(3) responsiveness have a clear genetic basis. Due to the percentage of the total variance explained, they are good candidates to participate in the genetic determination of the cluster architecture.