Cathepsin L: Critical role in Ii degradation and CD4 T cell selection in the thymus
Cathepsin L: Critical role in Ii degradation and CD4 T cell selection in the thymus
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DOI：
10.1126/science.280.5362.450
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发表时间：
1998-04-17
期刊：
影响因子：
56.9
通讯作者：
Rudensky, AY
中科院分区：
文献类型：
--
作者：
Nakagawa, T;Roth, W;Rudensky, AY
不变链（li）的降解是主要组织相容性复合物II类限制性抗原呈递的关键步骤。组织蛋白酶L被发现是必需的皮质胸腺上皮细胞（cTECs），但不是在骨髓（BM）衍生的抗原呈递细胞（APC）的锂降解。因此，CD4（+）T细胞的阳性选择减少。由于不同的半胱氨酸蛋白酶负责cTEC和BM衍生的APC中的特定li降解步骤，因此介导阳性和阴性选择的细胞中的蛋白水解环境可能是不同的。以组织特异性方式参与II类呈递的蛋白酶的鉴定表明了在体内操纵CD4（+）T细胞反应性的潜在方法。
Degradation of invariant chain (li) is a critical step in major histocompatibility complex class II-restricted antigen presentation. Cathepsin L was found to be necessary for li degradation in cortical thymic epithelial cells (cTECs), but not in bone marrow (BM)derived antigen-presenting cells (APCs). Consequently, positive selection of CD4(+) T cells, was reduced. Because different cysteine proteinases are responsible for specific li degradation steps in cTECs and BM-derived APCs, the proteolytic environment in cells mediating positive and negative selection may be distinct. The identification of a protease involved in class II presentation in a tissue-specific manner suggests a potential means of manipulating CD4(+) T cell responsiveness in vivo.