THROMBOSTHENIN-A CONTRACTILE PROTEIN FROM THROMBOCYTES - ITS EXTRACTION FROM HUMAN BLOOD PLATELETS AND SOME OF ITS PROPERTIES
THROMBOSTHENIN-A CONTRACTILE PROTEIN FROM THROMBOCYTES - ITS EXTRACTION FROM HUMAN BLOOD PLATELETS AND SOME OF ITS PROPERTIES
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DOI：
10.1016/0006-3002(61)90250-5
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发表时间：
1961-01-01
期刊：
影响因子：
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通讯作者：
LUSCHER, EF
中科院分区：
文献类型：
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作者：
BETTEXGALLAND, M;LUSCHER, EF
在用0.6[图片] KCl缓冲溶液提取人血小板时，得到一种蛋白质组分，其在离子强度低于0.2时变得不溶。如果存在ATP，这种材料将显示超沉淀或主动收缩的现象。由于其在血小板功能中的作用，我们将这种收缩蛋白命名为血栓素。与肌肉中的肌动球蛋白一样，血栓素也是一种ATP酶。这种酶的活性的依赖性上的离子强度，上的浓度的Ca 2+和Mg 2+离子，和ATP已被调查。测定了血栓素溶液对ATP的敏感性。虽然得到的结果表明，血栓蛋白属于肌动球蛋白组，定量比较与肌肉肌动球蛋白的数据导致的结论，血栓蛋白是不同的收缩蛋白的肌肉。血小板中血栓素的存在有关的粘性变形和凝块收缩的意义进行了讨论。
On extraction of human blood platelets with a buffered 0.6[image] KC1 solution, a protein fraction is obtained, which becomes insoluble at ionic strengths below 0.2. If ATP is present, this material will show the phenomenon of superprecipitation or active contraction. Because of its role in thrombocyte function, we have named this contracile protein thrombosthenin. Like actomyosin from muscle, thrombosthenin acts as an ATP-ase. The dependence of this enzymic activity on the ionic strength, on the concentrations of Ca2+ and Mg2+ ions, and of ATP has been investigated. The sensitivity of solutions of thrombosthenin towards ATP has been determined. Although the obtained results suggest that thrombosthenin belongs to the actomyosin group, a quantitative comparison with the data on muscle actomyosin leads to the conclusion, that thrombosthenin is distinct from the contractile protein of muscle. The significance of the presence of thrombosthenin in blood platelets in relation to viscous metamorphosis and clot retraction is discussed.