Endoscopic imaging and size estimation of colorectal adenomas in the multiple intestinal neoplasia mouse
Endoscopic imaging and size estimation of colorectal adenomas in the multiple intestinal neoplasia mouse
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DOI：
10.1016/j.gie.2008.09.054
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发表时间：
2009-03-01
影响因子：
7.7
通讯作者：
Clapper, Margie L.
中科院分区：
文献类型：
--
作者：
Hensley, Harvey H.;Merkel, Carrie E.;Clapper, Margie L.
背景资料：由于我们在体内监测肿瘤生长的能力有限，因此尚未充分认识到致癌动物模型的科学潜力。目的：开发一种基于内窥镜的方案，用于根据结肠镜检查期间获得的图像准确估计体内腺瘤大小。设计：比较内窥镜检查期间获得的病变大小估计值与磁共振成像（MRI）和尸检获得的病变大小估计值。设置：小动物成像设施。主题：腺瘤性息肉病结肠多发性肠化生Fox Chase癌症中心小鼠，患有多发性结直肠腺瘤。方法：小鼠通过使用刚性内窥镜接受结肠镜检查，并获得结肠腺瘤的高分辨率图像通过使用电荷耦合器件相机捕获。通过使用一种新的几何结构，比较腺瘤相对于参考杆的尺寸，估计病变大小。主要结果测量：结肠腺瘤的横截面积结果：在结肠镜检查过程中，在体内测量了20个腺瘤的横截面积，并与尸检时测量的大小进行比较，得出Pearson相关系数为0.94（P = 6.52 x 10（-9））。当使用11个腺瘤的测量值时，对手术操作者的变异性进行评估，得出Pearson相关系数为0.85（P = 4.35 x 10（-3）），并显示出良好的可重复性。局限性：只能观察远端结肠，内窥镜测量是二维的。结论：建立了一种可靠的体内结肠直肠腺瘤测量的内窥镜方法。应用这项技术的小鼠模型的结肠癌将提供独特的见解腺瘤生长的动力学。（Gastrointest Endosc 2009;69：742-9.）
Background: The scientific potential of animal models of carcinogenesis has not been fully realized because of our limited ability to monitor tumor growth in vivo.Objective: To develop an endoscopy-based protocol for the accurate estimation of adenoma size in vivo from images obtained during colonoscopy.Design: To compare estimates of lesion size acquired during endoscopy with those obtained from magnetic resonance imaging (MRI) and at necropsy.Setting: A small-animal imaging facility.Subjects: Adenomatous polyposis coli multiple intestinal metaplasia Fox Chase Cancer Center mice that develop multiple colorectal adenomas.Methods: The mice received colonoscopic examination by using a rigid endoscope, and high-resolution images of colon adenomas were captured by using a charge-coupled-device camera. Lesion size vas estimated by comparing the dimensions of the adenoma relative to a reference rod by using a novel geometric construction. The resulting areas were compared with estimates from MRIs and validated at necropsy.Main Outcome Measurements: Cross-sectional area of colon adenomas.Results: The cross-sectional area of 20 adenomas was measured in vivo during colonoscopy and compared with the size as measured at necrospy, which yielded a Pearson correlation coefficient of 0.94 (P = 6.52 x 10(-9)). Assessment of interoperator variaibility when using measurements from 11 adenomas, yielded a Pearson correlation coefficient of 0.85 (P = 4.35 x 10(-3)) and demonstrated excellent reproducibility.Limitations: Only the distal colon could be viewed, and endoscopic measurements were 2-dimensional.Conclusions: An endoscopic method for the reliable measurement of colorectal adenomas in vivo was established. The application of this technique to mouse models of colon carcinogenesis will provide unique insight into the dynamics of adenoma growth. (Gastrointest Endosc 2009;69:742-9.)