Zearalenone exposure affects mouse oocyte meiotic maturation and granulosa cell proliferation
Zearalenone exposure affects mouse oocyte meiotic maturation and granulosa cell proliferation
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DOI：
10.1002/tox.21995
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发表时间：
2015-09-01
影响因子：
4.5
通讯作者：
Sun, Shao-Chen
中科院分区：
文献类型：
--
作者：
Hou, Yan-Jun;Zhu, Cheng-Cheng;Sun, Shao-Chen
玉米赤霉烯酮（ZEN）是镰刀菌的代谢产物，是谷物和食品的常见污染物。ZEN作为一种异种雌激素，被认为具有细胞毒性、组织毒性和遗传毒性，可导致人类和动物流产和死胎。由于雌激素在减数分裂过程中影响卵母细胞的成熟，在这项研究中，我们研究了ZEN对小鼠卵母细胞减数分裂成熟和颗粒细胞增殖的影响。结果表明，ZEN处理的卵母细胞成熟率降低，这可能是由于细胞骨架的破坏：（1）ZEN处理导致更多的卵母细胞具有异常的纺锤体形态;（2）ZEN处理后肌动蛋白丝的表达和分布也受到破坏，这通过肌动蛋白调节蛋白的异常分布得到证实。此外，ZEN处理后皮质无颗粒结构域（CGFD）被破坏，这表明ZEN可能影响小鼠卵母细胞受精能力。MTT法和TUNEL凋亡分析表明，ZEN以剂量依赖的方式降低小鼠颗粒细胞增殖，这可能是卵母细胞成熟降低的另一个原因。因此，我们的研究结果表明，暴露于玉米赤霉烯酮影响小鼠卵母细胞减数分裂成熟和颗粒细胞增殖。(c)2014 Wiley Periodicals，Inc.环境毒理学30：1226-1233，2015年。
Zearalenone (ZEN) is a metabolite of Fusarium and is a common contaminant of grains and foodstuffs. ZEN acts as a xenoestrogen and is considered to be cytotoxic, tissue toxic, and genotoxic, which causes abortions and stillbirths in humans and animals. Since estrogens affect oocyte maturation during meiosis, in this study we investigated the effects of ZEN on mouse oocyte meiotic maturation and granulosa cell proliferation. Our results showed that ZEN-treated oocyte maturation rates were decreased, which might be due to the disrupted cytoskeletons: (1) ZEN treatment resulted in significantly more oocytes with abnormal spindle morphologies; (2) actin filament expression and distribution were also disrupted after ZEN treatment, which was confirmed by the aberrant distribution of actin regulatory proteins. In addition, cortical granule-free domains (CGFDs) were disrupted after ZEN treatment, which indicated that ZEN may affect mouse oocyte fertilization capability. ZEN reduced mouse granulosa cell proliferation in a dose-dependent manner as determined by MTT assay and TUNEL apoptosis analysis, which may be another cause for the decreased oocyte maturation. Thus, our results demonstrated that exposure to zearalenone affected oocyte meiotic maturation and granulosa cell proliferation in mouse. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 1226-1233, 2015.