Developmental biology using purified genes
Developmental biology using purified genes
复制标题
使用纯化基因的发育生物学
DOI：
10.1038/nm.2929
复制
发表时间：
2012
期刊：
影响因子：
82.9
通讯作者：
Donald D. Brown
中科院分区：
文献类型：
--
作者：
Donald D. Brown
1959 年，即 Lac 阻遏蛋白被发现的第二年，我从 NIH 前往巴黎巴斯德研究所研究细菌基因调控。在离开贝塞斯达之前，我非常幸运地了解到巴尔的摩有一个小型研究机构，当时与约翰·霍普金斯医学院有联系，称为华盛顿卡内基研究所胚胎学系。我联系了主任吉姆·艾伯特，并在巴黎一年后安排了高级博士后奖学金。很难想象有两个更加多元化的研究机构。巴斯德研究所处于生物学的最前沿，参与了分子生物学的创立。每天午餐时，雅克·莫诺 (Jacques Monod)、弗朗索瓦·雅各布 (François Jacob) 和安德烈·利沃夫 (André Lwoff) 都会主持一场激动人心的讨论，通常还会有一位知名访客进行补充。胚胎学系刚刚结束了 50 年来描述人类胚胎解剖发育的过程。该教师主要从事实验胚胎学。我是第一位生物化学家。这就是我花了 50 年时间在巴尔的摩卡内基研究所胚胎学系研究发育中的基因调控这一主题的原因。我无法想象还有比这更理想的地方了。
From the NIH I went to the Pasteur Institute in Paris to study bacterial gene regulation in 1959, the year after the Lac repressor had been discovered. Before leaving Bethesda, by the greatest luck I learned about a small research institution in Baltimore that was associated at that time with the Johns Hopkins Medical School called the Department of Embryology of the Carnegie Institution of Washington. I contacted Jim Ebert, the director, and arranged an advanced postdoctoral fellowship after my year in Paris. It is hard to imagine two more diverse research institutions.The Pasteur Institute was at the forefront of biology, involved in the founding of molecular biology. Every day at lunch Jacques Monod, François Jacob and André Lwoff presided over an exciting discussion usually augmented by a prominent visitor. The Department of Embryology was just phasing out 50 years of describing the anatomical development of the human embryo. The faculty was principally engaged in experimental embryology. I was the first biochemist. This is how I came to spend 50 years pursuing the topic of gene regulation in development at the Carnegie Institution’s Department of Embryology in Baltimore. I cannot imagine a more ideal place.