[Freeze fracturing and thin-section study of intercellular junctions between odontoblasts and the rat incisor. Changes induced by vinblastine].
[Freeze fracturing and thin-section study of intercellular junctions between odontoblasts and the rat incisor. Changes induced by vinblastine].
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[成牙本质细胞与大鼠门牙细胞间连接的冷冻断裂和薄片研究。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
D. Septier
中科院分区：
文献类型：
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作者：
M. Goldberg;F. Escaig;D. Septier
冻裂后的切片和复制品的研究表明，幼龄分泌成牙细胞的细胞间连接分为两种类型。粘着小带（桥粒）在牙前边界将细胞体连接在一起。在这些结构的前面观察到间隙连接。注射硫酸长春碱引起4h， a)间隙连接之间的膜间间隙扩大，b)桥粒接触表面减少，c)与连接复合体相关的由微丝组成的胞浆内致密物质增厚。这些修饰有利于在冷冻断裂后观察这些结构。桥粒连接可以发挥机械功能（分隔牙前腔室），而间隙连接允许成牙细胞的电紧张偶联。然而，它们允许离子成分（钙）和血清来源的基质成分的细胞间扩散途径。
The study of thin sections and replicas obtained after freeze-fracture showed that the intercellular junctions of the young secretory odontoblasts of the rat incisor were of two types. Zonula adherens (desmosomes) united the cellular bodies at the predentinal border. Ahead of these structures gap junctions were observed. An injection of vinblastine sulfate induced 4h, a) an enlargement of intermembranous spaces between the gap junctions, b) a reduction of the contact surfaces in the desmosomes and c) a thickening of the intracytoplasmic dense material made of microfilaments, associated with the junctional complex. These modifications favoured the observation of these structures after freeze-fracture. The desmosome junctions could play a mechanical function (partitioning of the predentinal compartment) whereas the gap junction allowed the electrotonic coupling of the odontoblasts. They allowed however an intercellular diffusion route for ionic components (calcium) and matrix components of serum origin.