Aliicoccus persicus gen. nov., sp nov., a halophilic member of the Firmicutes isolated from a hypersaline lake
Aliicoccus persicus gen. nov., sp nov., a halophilic member of the Firmicutes isolated from a hypersaline lake
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DOI：
10.1099/ijs.0.058545-0
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发表时间：
2014-06-01
影响因子：
2.8
通讯作者：
Ventosa, Antonio
中科院分区：
文献类型：
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作者：
Amoozegar, Mohammad Ali;Bagheri, Maryam;Ventosa, Antonio
从伊朗超盐湖 Aran-Bidgol 的盐水样本中分离出一种新型革兰氏染色阳性、中度嗜盐细菌，命名为菌株 A76(T)。细胞严格需氧、球菌状、不活动、不形成孢子、过氧化氢酶和氧化酶呈阳性。菌株 A76(T) 在 pH 7.0 至 10.0（pH 8.0 时最佳生长）、20 至 45°C（35°C 最佳生长）和 0.5 至 12.5% (w/v) NaCl 盐度（7.5%，w/v，NaCl 最佳生长）下生长。 16S rRNA基因序列分析表明，菌株A76(T)属于厚壁菌门，与Jeotgalicoccus、Salinicoccus和Noscomiicoccus属的模式种的序列相似性分别为94.1%、93.1%和91.1%。该新分离株的 DNA G+C 含量为 38.8 mol%。菌株A76(T)的主要细胞脂肪酸为anteiso-C-15:0和iso-C-15:0，其极性脂质模式由二磷脂酰甘油、磷脂酰甘油、一种糖脂、一种未知脂质和两种未知磷脂组成。类异戊二烯醌为MK-6 (940/)、MK-5 (3%) 和MK-7 (3%)。细胞壁的氨基酸成分为 Lys、Asp、Gly、Glu 和 Ala。菌株 A76(T) 与已有效公布名称的分类群模式菌株之间的生理、生化和系统发育差异表明，该菌株代表了葡萄球菌科新属中的新种，该属被命名为 Aliicoccus persicus gen。十一月，SP。十一月被提议。白粉阿里球菌的模式菌株是菌株A76(T)(＝CECT 8508(T)＝DSM 28306(T)＝IBRC-M 10081(T))。
A novel Gram-staining-positive, moderately halophilic bacterium, designated strain A76(T), was isolated from a brine sample of the hypersaline lake Aran-Bidgol in Iran. Cells were strictly aerobic, coccus-shaped, non-motile, non-sporulating, and catalase- and oxidase-positive. Strain A76(T) grew between pH 7.0 and 10.0 (optimal growth at pH 8.0), between 20 and 45 degrees C (optimal growth at 35 degrees C) and at salinities of 0.5 to 12.5% (w/v) NaCl (optimal growth at 7.5%, w/v, NaCl). On the basis of 16S rRNA gene,sequence analysis, strain A76(T) was shown to belong to the phylum Firmicutes with sequence similarities of 94.1, 93.1 and 91.1%, to the type species of the genera Jeotgalicoccus, Salinicoccus and Nosocomiicoccus, respectively. The DNA G+C content of this new isolate was 38.8 mol%. The major cellular fatty acids of strain A76(T) were anteiso-C-15:0 and iso-C-15:0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, an unknown lipid and two unknown phospholipids. The isoprenoid quinones were MK-6 (940/), MK-5 (3%) and MK-7 (3%). The amino acid constituents of the cell wall were Lys, Asp, Gly, Glu and Ala. The physiological, biochemical and phylogenetic differences between strain A76(T) and type strains of taxa with validly published names suggest that this strain represents a novel species in a novel genus within the family Staphylococcaceae, for which the name Aliicoccus persicus gen. nov., sp. nov. is proposed. The type strain of Aliicoccus persicus is strain A76(T) (=CECT 8508(T)=DSM 28306(T)=IBRC-M 10081(T)).