Genetic characterization of pear varieties revealed by chloroplast DNA sequences
Genetic characterization of pear varieties revealed by chloroplast DNA sequences
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DOI：
10.1080/14620316.2003.11511612
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发表时间：
2003-03-01
影响因子：
1.9
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Kimura, T;Iketani, H;Yamamoto, T
鉴定了 5 个物种的 8 个梨品种的 cpDNA（叶绿体 DNA）、atpB-rbcL 间隔区、trnL-trnF 间隔区、accD-psal 间隔区、ndhA 内含子、rp116 内含子和 rpoC1 内含子的 6 个非编码区的核苷酸序列。在超过5.7 kbp的核苷酸序列中共发现了38个核苷酸取代、缺失和插入等突变。基于突变的分支图显示了四种类型，包括一个欧洲梨组和三个亚洲梨组。揭示了 33 个梨品种的 trnL-trnF 核苷酸序列，并鉴定了 8 个突变。从数据中获得的分支图显示，亚洲梨品种分为六组，亚洲梨存在种内和种间多样性，而欧洲梨品种在 trnL-trnF 区域方面是相同的。使用 trnL-trnF 序列对来自种间杂交的八个梨品种进行了母性分析。四个品种显示出与其所描述的母本不同的核苷酸序列，这表明母本和父本可能存在反向组合。两个杂交品种与其母本之间不存在矛盾。
Nucleotide sequences at six noncoding regions of cpDNA (chloroplast DNA), atpB-rbcL spacer, trnL-trnF spacer, accD-psaI spacer, ndhA intron, rp116 intron and rpoC1 intron, were identified for eight pear varieties from five species. A total of 38 mutations such as nucleotide substitution, deletion and insertion were found in more than 5.7 kbp of nucleotide sequences. A cladogram based on the mutations showed four types including one European pear group and three Asian pear groups. Nucleotide sequences at the trnL-trnF were revealed for 33 pear varieties and eight mutations were identified. A cladogram obtained from the data showed that Asian pear varieties were divided into six groups and that intraspecific as well as interspecific diversities existed in Asian pears, whereas European pear varieties were identical with respect to the trnL-trnF region. Maternity analysis of eight pear varieties derived from interspecific crosses was conducted using the trnL-trnF sequences. Four varieties showed different nucleotide sequences from their described female parents, suggesting the possibility of reverse combinations of female and male parents. There was no contradiction between two hybrid varieties and their female parent.