FERTILIZATION STUDIES IN THE HAMSTER - THE ROLE OF CELL-SURFACE CARBOHYDRATES
FERTILIZATION STUDIES IN THE HAMSTER - THE ROLE OF CELL-SURFACE CARBOHYDRATES
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DOI：
10.1016/0014-4827(82)90124-0
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发表时间：
1982-01-01
影响因子：
3.7
通讯作者：
AHUJA, KK
中科院分区：
文献类型：
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作者：
AHUJA, KK
仓鼠配子表面上的互补结合位点的性质进行了分析，在体外系统中使用的单糖和寡糖，糖蛋白和糖苷酶。获能精子的比宁的透明质酸被抑制的几个单和寡糖有关的岩藻糖，半乳糖和乙酰化氨基糖，但不无关的糖。几种糖蛋白与假体碳水化合物基团丰富或终止于半乳糖或N-乙酰葡糖胺残基也是有效的受精抑制剂。在所有测试的糖蛋白中，2种血浆糖蛋白，α 1-酸性糖蛋白（乳清类粘蛋白）和胎球蛋白是最有效的。在它们的天然形式中，它们是非抑制性的，但它们的去唾液酸化（半乳糖终止）形式完全阻止精子结合。具有N-乙酰氨基葡萄糖末端的无半乳糖-orosomucoid也抑制受精。用α-γ射线处理获能精子L-岩藻糖苷酶，α- D-半乳糖苷酶和β- N-乙酰氨基己糖苷酶完全抑制受精作用，而其他糖苷酶、胰蛋白酶和芳基硫酸酯酶则不起作用。抑制性的谷胱甘肽和谷胱甘肽酶不干扰精子运动，对精子-卵膜融合也没有影响。用这些试剂预处理无卵丘卵母细胞也不抑制精子透明质酸结合。这些结果提供了证据，精子-透明质酸结合是由精子表面含有岩藻糖，半乳糖，N-乙酰葡萄糖胺和N-乙酰半乳糖胺残基的配体介导的。
The nature of complementary binding sites on the surfaces of hamster gametes was analyzed using mono- and oligosaccharides, glycoproteins and glycosidases in an in vitro system. The bining of capacitated spermatozoa to the zona pellucida was inhibited by several mono- and oligosaccharides related to fucose, galactose and acetylated amino sugars, but not by unrelated sugars. Several glycoproteins with prosthetic carbohydrate groups rich in or terminated by galactose or N-acetylglucosamine residues were also potent inhibitors of fertilization. Of all the glucoproteins tested, 2 plasma glycoproteins, .alpha.1-acid glycoproteins (orosomucoid) and fetuin were most effective. In their native form they were non-inhibitory but their desialylated (galactose-terminated) forms completely prevented the sperm-zona binding. Agalacto-orosomucoid with N-acetylglucosamine terminals also inhibited fertilization. The treatment of capacitated spermatozoa with .alpha.-L-fucosidase, .alpha.-D-galactosidase and .beta.-N-acetylhexosaminidase, but not with other glycosidases, trypsin and arylsulfatase, reulted in the complete inhibition of fertilization. Inhibitory saccharides and glycosidases did not interfere with sperm motility and had no effect on sperm-oolemma fusion. The pretreatment of cumulus-free oocytes with these agents did not inhibit sperm zona pellucida binding either. These results provide evidence that sperm-zona pellucida binding is mediated by ligands on the sperm surface containing fucose, galactose, N-acetylglucosamine and N-acetylgalactosamine residues.