Potentiation of the immunotoxicity of ethanol by acetaminophen in mice
Potentiation of the immunotoxicity of ethanol by acetaminophen in mice
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DOI：
10.1016/s1567-5769(01)00121-7
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发表时间：
2002-01-01
影响因子：
5.6
通讯作者：
Park, JS
中科院分区：
文献类型：
--
作者：
Kim, JH;Park, JS
为探讨对乙酰氨基酚(APP)对乙醇(EtoH)免疫毒性的影响，给ICR小鼠灌胃APP(100 mg/kg)，连续28天。在实验期间，乙醇处理的小鼠免费给予20%w/v乙醇，正常小鼠则给予赋形剂。本研究结果如下：APP与EtoH联用较单用EtoH能显著降低小鼠外周血白细胞数及肝、脾、胸腺的相对重量。在AAP和EtoH联合治疗的小鼠中，与EtoH单独治疗相比，小鼠的脾斑块形成细胞(PFC)和对绵羊红细胞(SRBC)的血凝(HA)滴度以及对牛血清白蛋白(BSA)的二次抗体应答也显著降低。在AAP和EtoH的联合应用中，也观察到抑制迟发型超敏反应(DTH)和吞噬活性的趋势。此外，与乙醇单独治疗相比，AAP和乙醇联合应用可显著提高血清丙氨酸氨基转移酶(ALT)和总蛋白水平。血清白蛋白和A/G比值在给予乙醇的小鼠显著低于正常动物，而给予AAP和EtoH的小鼠则进一步降低。这些结果表明，APP和EtoH联合应用可加重Etoh诱导的免疫毒性。(C)2002 Elsevier Science B.V.保留所有权利。
To investigate the influences of acetaminophen (APP) on the immunotoxicity of ethanol (EtOH) in ICR mice, APP at a dose of 100 mg/kg was orally administered to mice daily for 28 consecutive days. Mice treated with EtOH were given freely with 20% w/v EtOH during the experimental period, and normal mice were given vehicle. The results of this study are summarized as follows: the combination of APP and EtOH significantly decreased the circulating leukocytes and the relative weights of liver, spleen and thymus, compared with the treatment of EtOH alone. In mice receiving the combination of AAP and EtOH when compared with the treatment of EtOH alone, there were also significant reductions in the splenic plaque forming cells (PFC) and hemagglutination (HA) titers to sheep red blood cells (SRBC), and the secondary IgG antibody response to bovine serum albumin (BSA). A tendency toward suppression of delayed-type hypersensitivity (DTH) reaction and phagocytic activity was also observed in the combination of AAP and EtOH. In addition, the combination of AAP and EtOH greatly increased serum alanine aminotransaminase (ALT) and total protein levels, compared with the treatment of EtOH alone. Significant decreases in serum albumin and A/G ratio were observed in EtOH alone-fed mice compared with those in normal animals, and their reductions were further induced in mice treated with AAP and EtOH. These findings indicate that EtOH-induced immunotoxicity is aggravated by the combination of APP and EtOH. (C) 2002 Elsevier Science B.V. All rights reserved.