3H-Estradiol: In Vitro Binding to Macromolecules from the Rat Hypothalamus, Anterior Pituitary and Uterus
3H-Estradiol: In Vitro Binding to Macromolecules from the Rat Hypothalamus, Anterior Pituitary and Uterus
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3H-雌二醇：体外与大鼠下丘脑、垂体前叶和子宫的大分子结合
DOI：
10.1210/endo-86-6-1313
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发表时间：
1970
期刊：
影响因子：
4.8
通讯作者：
A. Eisenfeld
中科院分区：
文献类型：
--
作者：
A. Eisenfeld
本文研究了~ 3 H-雌二醇与不同器官大分子的体外相互作用。将卵巢切除大鼠的组织在含EDTA和放射性雌二醇的Tris-HCl中与100，000 × g上清液混合匀浆。孵育后，通过凝胶过滤分离大分子结合的放射性。在下丘脑、垂体前叶和子宫的大分子组分中发现的放射性雌二醇浓度高于大脑、小脑、心脏或血浆。发现来自下丘脑、垂体前叶和子宫的结合分子具有以下性质。非放射性雌二醇（10− 7 m）会减少结合，而孕酮或睾酮（10− 5 m）则不会。静脉注射非放射性雌二醇可降低体外3 H-雌二醇的后续结合。结合分子含有蛋白质，似乎具有影响与雌二醇相互作用的巯基。（内分泌学86：1313，1970）
The in vitro interaction of 3H-estradiol with macromolecules from various organs was studied. Tissues from ovariectomized rats were homogenized in Tris-HCl containing EDTA and radioactive estradiol mixed with the 100,000 × g supernatants. After incubation macromolecular bound radioactivity was separated by gel filtration. Higher concentrations of radioactive estradiol were found in the macromolecular fractions from the hypothalamus, anterior pituitary and uterus than from the cerebrum, cerebellum, heart or plasma. The binding molecules from the hypothalamus, anterior pituitary and uterus were found to have the following properties. Nonradioactive estradiol (10−7m) reduced binding, whereas progesterone or testosterone (10−5m) did not. Intravenous injection of nonradioactive estradiol decreased subsequent binding of 3H-estradiol in vitro. The binding molecules contain protein and appear to have sulfhydryl groups that influence the interaction with estradiol. (Endocrinology 86: 1313, 1970)