A colorimetric sensing platform based upon recognizing hybridization chain reaction products with oligonucleotide modified gold nanoparticles through triplex formation
A colorimetric sensing platform based upon recognizing hybridization chain reaction products with oligonucleotide modified gold nanoparticles through triplex formation
复制标题
基于通过三链体形成识别寡核苷酸修饰的金纳米颗粒的杂交链式反应产物的比色传感平台
DOI：
10.1039/c6nr09089c
复制
发表时间：
2017-02-07
期刊：
影响因子：
6.7
通讯作者：
Ling, Liansheng
中科院分区：
文献类型：
--
作者：
Zou, Li;Li, Ruimin;Ling, Liansheng
将杂交链反应（HCR）的信号放大与金纳米粒子（AuNPs）的三链体组装相结合，提出了一种新的生物分子比色检测方法。在靶点存在的情况下，HCR过程可以被触发，HCR产物被三链形成寡核苷酸（TFO）功能化的金纳米颗粒特异性识别，伴随着金纳米颗粒的聚集和522 nm处显着的吸光度变化。此外，DNA发夹探针可以在不存在靶标的情况下与TF0官能化的AuNP形成刚性三链体结构，导致可忽略的背景信号。利用这种新的生物传感器平台，广泛的目标，包括核酸，小分子和蛋白质，已成功地确定了高灵敏度和选择性，这可能具有巨大的实际应用潜力。
A novel colorimetric sensing strategy for biomolecule assay has been developed, which integrates the signal amplification of the hybridization chain reaction (HCR) with the assembly of gold nanoparticles (AuNPs) through triplex formation. In the presence of targets, the HCR process can be triggered, the HCR products are specifically recognized by triplex-forming oligonucleotide (TFO) functionalized AuNPs, accompanying the aggregation of AuNPs and a dramatic absorbance change at 522 nm. In addition, the DNA hairpin probes can form rigid triplex structures with TFO-functionalized AuNPs in the absence of targets, resulting in a negligible background signal. By taking advantage of this new biosensor platform, a broad range of targets, involving nucleic acids, small molecules and proteins, have been successfully determined with high sensitivity and selectivity, which may hold great potential for practical application.