Alternate release of different target species based on the same gold nanorods and monitored by cell imaging
Alternate release of different target species based on the same gold nanorods and monitored by cell imaging
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基于相同金纳米棒并通过细胞成像监测的不同目标物质的交替释放
DOI：
10.1016/j.colsurfb.2016.05.087
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发表时间：
2016
影响因子：
5.8
通讯作者：
Liu Xuanyong
中科院分区：
文献类型：
--
作者：
Chen Shenna;Huang Haowen;Zhang Lingyang;Chen Yun;Liu Xuanyong
在这项研究中，开发了一种不同类型的分子在同一金纳米棒(GNR)上加载和释放的策略。首次将抗癌药物盐酸阿霉素(DOX)化学偶联到GNRs上。为了有效地将另一种类型的靶分子DNA负载到相同的GNR上，将聚电解质聚(乙烯亚胺)(PEI)吸附在GNR@DOX上形成GNR@DOX@PEI。然后，正电荷GNR@DOX@PEI允许GNR偶联物通过静电相互作用与带负电荷的DNA相互作用，使它们完全接合。制作了一个平台，可以负载两种共轭在同一GNRs上的靶分子。另一方面，化学反应和近红外(NIR)激光可以触发不同目标物种的选择性和顺序释放。DOX的释放是通过Na2S2O3与GNRs的反应实现的，而DNA在GNR@DOX@PEI上的释放是通过近红外激光局部加热实现的。此外，通过荧光成像监测了不同靶物质从MCF-7细胞内的GNRs选择性和交替释放，提供了一种潜在的协同癌症治疗。
In this study, a strategy for load and release of different kinds of molecules on the same gold nanorods (GNRs) was developed. An anticancer drug, doxorubicin hydrochloride (DOX), was firstly chemically conjugated on the GNRs. To efficiently load another type of target molecules DNA on the same GNRs, a polyelectrolyte Poly (ethylene imine) (PEI) was adsorbed on the GNR@DOX to form GNR@DOX@PEI. Then, the positive charge GNR@DOX@PEI allows the GNR conjugates to interact with negative charged DNA by an electrostatic interaction, enabling their full conjugation. A platform to load two kinds of target molecules conjugated on the same GNRs was fabricated. On the other hand, selective and sequential release of the different target species may be triggered by chemical reaction and near infrared (NIR) laser. The release of DOX was achieved by Na2S2O3reacting with GNRs and the discharge of DNA conjugated on the GNR@DOX@PEI was accomplished by local-heating using NIR laser triggered release. Furthermore, the selective and alternate release of different target species from the GNRs inside MCF-7 cells was monitored by fluorescent imaging, providing a potential synergistic cancer treatment.