Acupuncture Induces the Proliferation and Differentiation of Endogenous Neural Stem Cells in Rats with Traumatic Brain Injury.
Acupuncture Induces the Proliferation and Differentiation of Endogenous Neural Stem Cells in Rats with Traumatic Brain Injury.
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针刺诱导脑外伤大鼠内源性神经干细胞增殖和分化
DOI：
10.1155/2016/2047412
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Lin H
中科院分区：
文献类型：
--
作者：
Jiang S;Chen W;Zhang Y;Zhang Y;Chen A;Dai Q;Lin S;Lin H
目的。目的 探讨针刺是否诱导创伤性脑损伤 (TBI) 大鼠模型中内源性神经干细胞 (NSC) 的增殖和分化。方法。 104只Sprague-Dawley大鼠随机分为正常组、模型组和针刺组。每组又分为三天（3 d）、七天（7 d）和十四天（14 d）组。采用Feeney自由落体硬膜外冲击法建立大鼠TBI模型。针刺组大鼠采用穴位（百会、水沟、风府、衙门、双侧合谷）进行治疗。正常组和模型组不接受针刺治疗。采用神经行为评分和苏木精-伊红染色评估大鼠TBI模型的建立和针刺的治疗效果。使用免疫荧光显微镜分析 TBI 大鼠中 NSC 的增殖和分化。结果。针刺组各时间点巢蛋白表达细胞、溴脱氧尿苷/胶质纤维酸性蛋白（BrdU/GFAP-）、BrdU/S100钙结合蛋白B阳性细胞、BrdU/微管相关蛋白2-和BrdU/半乳脑苷酶阳性细胞水平均较模型组显着升高（P < 0.01），但第 7 天和第 14 天的 BrdU/GFAP 阳性细胞。结论。针刺诱导NSCs增殖和分化，从而促进TBI大鼠的神经修复。
Purpose. To investigate whether acupuncture induced the proliferation and differentiation of endogenous neural stem cells (NSCs) in a rat model of traumatic brain injury (TBI). Methods. 104 Sprague-Dawley rats were randomly divided into normal, model, and acupuncture groups. Each group was subdivided into three-day (3 d), seven-day (7 d), and fourteen-day (14 d) groups. The rat TBI model was established using Feeney's freefall epidural impact method. The rats in the acupuncture group were treated at acupoints (Baihui, Shuigou, Fengfu, Yamen, and bilateral Hegu). The normal and model groups did not receive acupuncture. The establishment of the rat TBI model and the therapeutic effect of acupuncture were assessed using neurobehavioral scoring and hematoxylin-eosin staining. The proliferation and differentiation of NSCs in TBI rats were analyzed using immunofluorescence microscopy. Results. The levels of nestin-expressing cells and bromodeoxyuridine/glial fibrillary acidic protein- (BrdU/GFAP-) and BrdU/S100 calcium-binding protein B-positive and BrdU/microtubule-associated protein 2- and BrdU/galactocerebrosidase-positive cells were more significantly increased at various time points in the acupuncture group than in the model group (P < 0.01), except for a decreased level of BrdU/GFAP-positive cells at 7 d and 14 d. Conclusion. Acupuncture induced the proliferation and differentiation of NSCs, thereby promoting neural repair in the TBI rats.