Detection of aflatoxin M1 in milk products from China by ELISA using monoclonal antibodies
Detection of aflatoxin M1 in milk products from China by ELISA using monoclonal antibodies
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DOI：
10.1016/j.foodcont.2009.02.004
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发表时间：
2009-12-01
期刊：
影响因子：
6
通讯作者：
Lee, Won Jong
中科院分区：
文献类型：
--
作者：
Pei, Shi Chun;Zhang, Yuan Yuan;Lee, Won Jong
描述了一种使用单克隆抗体快速、灵敏的间接竞争酶联免疫吸附测定 (ELISA) 方法来测量牛奶和奶制品中的黄曲霉毒素 M1 (AFM1)。将骨髓瘤细胞与从用与牛血清白蛋白 (BSA) 缀合的 AFM1 羧甲基肟免疫的 BALB/c 小鼠分离的脾细胞融合后，分离出一种单克隆抗体并进行表征。抗 AFM1 单克隆抗体克隆对 AFM1、黄曲霉毒素 B1 (AFB1)、黄曲霉毒素 G1 (AFG1) 和脱氧雪腐镰刀菌烯醇 (DON) 的交叉反应性分别为 100%、13.9%、6.7% 和 < 1%。对与浓度范围为 0.1 至 3.2 ng/ml 的 AFM1 混合的牛奶样品进行分析，得出平均 ELISA 回收率为 98%。 AFM1的检测浓度限为0.04ng/ml。对从中国东北地区不同超市采集的12个原奶样品、15个奶粉样品、104个液态奶样品和4个奶酪样品进行了AFM1污染检测。在测试的 135 个牛奶样品中，55 个（41%）样品中 AFM1 含量为 0.32-0.50 ng/ml，24 个（18%）样品中含量为 0.16-0.32 ng/ml，18 个样品（13%）含量为 0-0.16 ng/ml；在 38 个 (28%) 样本中未检测到 AFM1。结果表明，必须采取必要的预防措施，尽量减少中国东北地区牛奶和奶制品中的 AFM1 污染。 (c) 2009 Elsevier Ltd. 保留所有权利。
A rapid and sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) method using monoclonal antibody for measuring aflatoxin M1 (AFM1) in milk and milk products has been described. One monoclonal antibody was isolated and characterized after fusion of myeloma cells with spleen cells isolated from BALB/c mice that had been immunized with AFM1 carboxymethyl oxime conjugated with bovine serum albumin (BSA). Cross-reactivities of the anti-AFM1 monoclonal antibody clone were 100, 13.9, 6.7 and < 1% against AFM1, aflatoxin B1 (AFB1), aflatoxin G1 (AFG1) and deoxynivalenol (DON), respectively. Assays of milk samples mixed with AFM1 ranging in concentration from 0.1 to 3.2 ng/ml gave mean ELISA recovery of 98%. The limit of detection concentration of AFM1 was 0.04 ng/ml. AFM1 contamination was measured in 12 samples of raw milk, 15 samples of powdered milk, 104 samples of liquid milk and four cheese samples collected from different supermarkets in Northeast of China. Of 135 milk samples tested, 55 (41%) samples contained AFM1 at levels that ranged from 0.32-0.50 ng/ml, 24 (18%) samples contained 0.16-0.32 ng/ml, and 18 (13%) samples contained 0-0.16 ng/ml; in 38 (28%) samples AFM1 was not detected. The results indicate that the necessary precaution will have to be taken to minimize the AFM1 contamination in milk and milk products from Northeast of China. (c) 2009 Elsevier Ltd. All rights reserved.