Mitotic activity in non-neoplastic melanocytes in vivo as determined by histochemical, autoradiographic, and electron microscope studies.
Mitotic activity in non-neoplastic melanocytes in vivo as determined by histochemical, autoradiographic, and electron microscope studies.
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由组织化学，放射自显微镜和电子显微镜研究确定的非肿瘤性黑素细胞的有丝分裂活性。
DOI：
10.1083/jcb.66.3.663
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发表时间：
1975-09
期刊：
影响因子：
--
通讯作者：
Szabo G
中科院分区：
文献类型：
--
作者：
Jimbow K;Roth SI;Fitzpatrick TB;Szabo G
在无外界刺激的情况下，正常表皮和非表皮组织分化的黑素细胞出现有丝分裂现象。这些红利黑素细胞存在于人和鼠的皮肤、鼠的毛、鸡的羽毛和鸡的胚胎视网膜色素上皮中。在正常成人的表皮中，分裂的黑素细胞虽然很少，但在非刺激区被发现。L-3，4-二羟基苯丙氨酸在黑素细胞有丝分裂过程中的反应证明了黑素形成酶和酪氨酸酶的活性，超微结构研究证明了不同成熟阶段特有的黑素小体。有丝分裂过程中黑素细胞的其他超微结构特征，除了高尔基体较小且较不复杂外，与分化良好的未分裂黑素细胞相似。胸腺嘧啶核苷掺入小鼠皮肤的放射自显影研究表明，当受到轻微刺激时，0.7%的表皮黑素细胞处于S时相。因此，非肿瘤性黑素细胞在体内的分化(产生酪氨酸酶和黑素小体)并不排除它们通过有丝分裂进行复制。
Mitotic figures were demonstrated in the differentiated melanocytes of normal epidermal and nonepidermal tissues without the presence of external stimuli. These dividine melanocytes were present in human and mouse skin, mouse hair, chick feathers, and embryonic chick retinal pigment epithelium. In normal adult human epidermis, dividing melanocytes, though rare, were found in the nonstimulated areas. L-3,4- dihydroxyphenylalanine reaction on the melanocytes during mitosis demonstrated activity of the melanin-forming enzyme, tyrosinase, and ultrastructural studies demonstrated the characteristic melanosomes in variour stages of maturation. Other ultrastructural characteristics of the melanocytes during mitosis, except for the Golgi apparatus, which was smaller and less complex, were similar to those seen in well- differentiated nondividing melanocytes. Autoradiographic studies of thymidine incorporation into mouse skin indicated that 0.7% of epidermal melanocytes, when slightly stimulated, are in the S phase. Thus, in vivo differentiation of non-neoplastic melanocytes (to produce pyrosinase and melanosomes) does not preclude their replication by mitotic division.