Characterizing and Modulating the Mechanical Properties of hydrogels from Ventricular Extracellular Matrix
Characterizing and Modulating the Mechanical Properties of hydrogels from Ventricular Extracellular Matrix
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心室细胞外基质水凝胶机械性能的表征和调节
DOI：
10.1109/ascc.2015.7244661
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Mitsuo Umezu
中科院分区：
文献类型：
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作者：
Kyohei Fujita;Yuuki Tuchida;Hiroki Seki;Daisuke Sato;Takao Nakamura;Tadashi Kosawada;Zhonggang Feng;Yasuyuki Shiraishi;Mitsuo Umezu
为了将多能干细胞分化为心肌细胞，最通用的方法是将干细胞暴露于与心脏发生相关的各种生长因子。然而，据报道，一种新方法可以在不补充生长因子的情况下诱导人 ES 细胞的心脏分化，即在由心脏细胞外基质 (ECM) 和 I 型胶原组成的混合凝胶中培养人 ES 细胞的胚状体。另一方面，支架的机械性能是干细胞分化的关键因素之一。然而，鉴于此和其他有关使用心脏 ECM 支架将干细胞分化为心肌细胞的报道，尚未彻底研究由心脏 ECM 制成的支架的机械性能。在本研究中，我们制备了由山羊心室细胞外基质组成的生物水凝胶，并通过单轴压缩试验研究了其力学性能。结果表明ECM凝胶具有粘弹性。这些凝胶在修正的非线性开尔文模型中的弹性模量 K1 为 9.5 Pa，K2 为 814.7 Pa。此外，通过使用 EDAC 进行化学处理，我们能够将弹性模量 K1 和 K2 分别提高到 139.7 Pa 和 2023.9 Pa。
In order to differentiate pluripotent stem cells to cardiomyocytes, the most general method is to expose stem cells to various growth factors related to cardiogenesis. However, a novel method has been reported to induce cardiac differentiation of human ES cells without supplemental growth factors by culturing embryoid body of human ES cells in hybrid gels composed of cardiac extracellular matrix (ECM) and type I collagen. On the other hand, mechanical properties of scaffold is one of the critical cue for differentiation of stem cells. However, it has not been thoroughly investigated the mechanical properties of the scaffold made from cardiac ECM in view of this and other reports about the differentiation of stem cells into cardiomyocytes using cardiac ECM scaffold. In this study, we fabricated bio-hydrogels composed of goat ventricular extracellular matrix, and investigated the mechanical properties by means of uniaxial compression test. It showed that the ECM gels possess viscoelastic property. The elastic modulus K1in modified non-linear Kelvin model is 9.5 Pa for these gels and K2is 814.7 Pa. Moreover, we were able to improve the elastic moduli K1and K2up to 139.7 Pa and 2023.9 Pa, respectively, by chemical treatment using EDAC.