Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder
Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder
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DOI：
10.1074/jbc.272.18.12008
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发表时间：
1997-05-02
影响因子：
4.8
通讯作者：
Diamond, G
中科院分区：
文献类型：
--
作者：
Cole, AM;Weis, P;Diamond, G
在许多脊椎动物和无脊椎动物的骨髓细胞和粘膜组织中发现了抗微生物肽，这些肽被预测作为第一线宿主防御机制发挥广谱活性以对抗病原性细菌、真菌、寄生虫和包膜病毒，我们报告了一种新的25个残基的线性抗菌肽中发现的皮肤粘液分泌物的冬比目鱼（美洲鲽）的表征。通过多种色谱方法对该肽进行了纯化，得到一个单一的峰，经细菌细胞裂解平板试验表明该肽具有抗菌活性，质谱和氨基酸序列分析表明该肽长25个氨基酸，命名为pleurocidin。预测Pleurocidin呈现类似于许多其它线性抗微生物肽的两亲性α-螺旋构象。pleurocidin和两种抗微生物肽（来自地中海果蝇的ceratotoxin和来自海蛙皮肤的dermaseptin）之间存在高度同源性，测定了pleurocidin对11种不同革兰氏阴性和革兰氏阴性细菌的最小抑菌浓度和最小杀菌浓度。阳性细菌，免疫组织化学定位pleurocidin在上皮粘液细胞的比目鱼皮肤，Pleurocidin代表了一种新的抗菌肽，发现在鱼类和可能发挥作用的先天宿主防御。
Antimicrobial peptides are found in both myeloid cells and mucosal tissues of many vertebrates and invertebrates, These peptides are predicted to operate as a first line host defense mechanism exerting broad-spectrum activity against pathogenic bacteria, fungi, parasites, and enveloped viruses, We report the characterization of a novel 25-residue linear antimicrobial peptide found in the skin mucous secretions of the winter flounder (Pleuronectes americanus). This peptide was purified through multiple chromatographic methods to obtain a single peak by reversed-phase high performance liquid chromatography, This purified peptide, which we named pleurocidin, exhibited antimicrobial activity against Escherichia coli in a bacterial cell lysis plate assay, Mass spectrometry and amino acid sequence analysis indicated that it is 25 amino acids in length. Pleurocidin is predicted to assume an amphipathic alpha-helical conformation similar to many other linear antimicrobial peptides, There is a high degree of homology between pleurocidin and two antimicrobial peptides, ceratotoxin from the Mediterranean fruit fly and dermaseptin from the skin of a hylid frog, The minimal inhibitory concentration and minimal bactericidal concentration of pleurocidin were determined against 11 different Gram-negative and Gram-positive bacteria, Immunohistochemistry locates pleurocidin in the epithelial mucous cells of flounder skin, Pleurocidin represents a novel antimicrobial peptide found in fish and may play a role in innate host defense.