Evolution of the hypoxia-sensitive cells involved in amniote respiratory reflexes
Evolution of the hypoxia-sensitive cells involved in amniote respiratory reflexes
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DOI：
10.7554/elife.21231
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发表时间：
2017-04-07
期刊：
影响因子：
7.7
通讯作者：
Baker, Clare V. H.
中科院分区：
文献类型：
--
作者：
Hockman, Dorik;Burns, Alan J.;Baker, Clare V. H.
引起呼吸反射的缺氧敏感细胞、颈动脉体球细胞和“肺神经内分泌细胞”（PNEC）的进化起源尚不清楚。神经嵴来源的血管球细胞与胚胎起源不明的鱼鳃缺氧敏感的神经上皮细胞（NEC）之间存在同源性。NEC也被比作PNEC，其在肺气道上皮内原位分化。使用遗传谱系追踪和神经嵴缺陷突变体的斑马鱼，和物理的命运映射在青蛙和七鳃鳗，我们发现，NECs不是神经嵴衍生的，但内胚层衍生的，像PNEC，其内胚层起源，我们确认。我们发现了与斑马鱼咽弓血管相关的神经嵴衍生的儿茶酚胺能细胞，并提出了一种新的模式，为斑马鱼的缺氧敏感细胞的演变：内胚层衍生的NECs被保留为PNEC，而颈动脉体的发展通过聚集的神经嵴衍生的儿茶酚胺能（嗜铬）细胞已经与血管在斑马鱼的咽弓。
The evolutionary origins of the hypoxia-sensitive cells that trigger amniote respiratory reflexes carotid body glomus cells, and 'pulmonary neuroendocrine cells' (PNECs) - are obscure. Homology has been proposed between glomus cells, which are neural crest-derived, and the hypoxia-sensitive 'neuroepithelial cells' (NECs) of fish gills, whose embryonic origin is unknown. NECs have also been likened to PNECs, which differentiate in situ within lung airway epithelia. Using genetic lineage-tracing and neural crest-deficient mutants in zebrafish, and physical fate mapping in frog and lamprey, we find that NECs are not neural crest-derived, but endoderm derived, like PNECs, whose endodermal origin we confirm. We discover neural crest-derived catecholaminergic cells associated with zebrafish pharyngeal arch blood vessels, and propose a new model for amniote hypoxia-sensitive cell evolution: endoderm-derived NECs were retained as PNECs, while the carotid body evolved via the aggregation of neural crest-derived catecholaminergic (chromaffin) cells already associated with blood vessels in anamniote pharyngeal arches.