The late retinoic acid induction of laminin B1 gene transcription involves RAR binding to the responsive element.
The late retinoic acid induction of laminin B1 gene transcription involves RAR binding to the responsive element.
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DOI：
10.1002/j.1460-2075.1991.tb08055.x
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发表时间：
1991-05
期刊：
影响因子：
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通讯作者：
G. Vasios;S. Mader;J. D. Gold;M. Leid;Y. Lutz;M. Gaub;P. Chambon;L. Gudas
中科院分区：
文献类型：
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作者：
G. Vasios;S. Mader;J. D. Gold;M. Leid;Y. Lutz;M. Gaub;P. Chambon;L. Gudas
鼠层粘连蛋白B1（LB 1）基因的转录是由维甲酸（RA）诱导的，但在F9 EC细胞中，RA处理后仅24-28 h才有反应。在这里，我们通过凝胶阻滞试验表明，Cos细胞中表达的所有三种视黄酸受体（RAR）α，β和γ都可以直接结合到先前表征的LB 1启动子的视黄酸反应元件（RARE）上，尽管亲和力比RAR-β基因RARE弱。三个立体排列的TGACC样基序对于这种结合至关重要。有趣的是，RAR-α、RAR-β和RAR-γ结合LB 1 RARE的能力似乎受到表达RAR的牛痘病毒载体感染的HeLa细胞中存在的因子的差异调节。对LB 1 RARE突变体的分析提供了体内RA诱导与体外RAR结合效率之间的强相关性。因此，RAR可以直接参与LB 1基因的转录诱导，即使这种诱导是放线菌酮敏感的和RAR存在于F9细胞RA添加之前。
Transcription of the murine laminin B1 (LB1) gene is induced by retinoic acid (RA), but responds only 24–28 h after RA treatment in F9 EC cells. Here we have shown by gel retardation assay that all three retinoic acid receptors (RARs) alpha, beta and gamma expressed in Cos cells can bind directly to the previously characterized retinoic acid response element (RARE) of the LB1 promoter, albeit with a weaker affinity than to the RAR‐beta gene RARE. Three stereo‐aligned TGACC‐like motifs are crucial for this binding. Interestingly, the capacity of RAR‐alpha, ‐beta and ‐gamma to bind the LB1 RARE appears to be differentially modulated by factor(s) present in HeLa cells infected with RAR‐expressing vaccinia virus vectors. Analyses of LB1 RARE mutants provide a strong correlation between RA‐inducibility in vivo and efficiency of RAR binding in vitro. Thus, RARs can participate directly in transcriptional induction of the LB1 gene, even though this induction is cycloheximide sensitive and RARs are present in F9 cells prior to RA addition.