HIV-SPECIFIC T-HELPER ACTIVITY IN SERONEGATIVE HEALTH-CARE WORKERS EXPOSED TO CONTAMINATED BLOOD
HIV-SPECIFIC T-HELPER ACTIVITY IN SERONEGATIVE HEALTH-CARE WORKERS EXPOSED TO CONTAMINATED BLOOD
复制标题
DOI：
10.1001/jama.271.1.42
复制
发表时间：
1994-01-05
影响因子：
120.7
通讯作者：
SHEARER, GM
中科院分区：
文献类型：
--
作者：
CLERICI, M;LEVIN, JM;SHEARER, GM
客观。-评估HIV血清阴性的医护人员与HIV感染（HIV阳性）患者的职业高风险暴露中人类免疫缺陷病毒（HIV）1型特异性细胞免疫应答。外周血单个核细胞（PBMC）后获得的职业暴露于HIV，和PBMC从卫生保健工作者暴露于HIV阴性患者作为对照。用HIV包膜合成肽体外刺激PBMC。在生物测定中测量白细胞介素2（IL-2）的产生。HIV抗体状态通过标准酶联免疫吸附试验测定。还通过聚合酶链反应技术对暴露个体进行了艾滋病毒前病毒DNA评估。对8名高危暴露的医护人员和9名对照医护人员的PBMC进行了研究。来自所有个体的PBMC显示出对对照抗原的强IL-2产生，表明完整的辅助性T细胞功能。8名HIV暴露者中有6名PBMC中检测到HIV多肽产生的白细胞介素2，而9名HIV阴性体液暴露者中只有1名PBMC中检测到HIV多肽产生的白细胞介素2（P
Objective.-To evaluate human immunodeficiency virus (HIV) type 1-specific cellular immune responses in HIV-seronegative health care workers with occupational high-risk exposures to HIV-infected (HIV-positive) patients.Design.-Peripheral blood mononuclear cells (PBMCs) were obtained after occupational exposures to HIV, and PBMCs from health care workers exposed to HIV-negative patients served as controls. The PBMCs were stimulated in vitro with HIV envelope synthetic peptides. Interleukin 2 (IL-2) production was measured in a bioassay. The HIV antibody status was determined by standard enzyme-linked immunosorbent assays. Exposed individuals were also evaluated for HIV proviral DNA by polymerase chain reaction techniques.Participants.-The PBMCs from eight health care workers with high-risk exposures and nine control health care workers were studied.Results.-The PBMCs from all individuals showed strong IL-2 production to control antigens, indicating intact T-helper function. Interleukin 2 production to HIV peptides was detected in PBMCs from six of eight HIV-exposed individuals, but in only one of the nine health care workers exposed to HIV-negative body fluids (P