Periodate oxidation studies in the elucidation of the structures of sialic acid containing oligosaccharides.
Periodate oxidation studies in the elucidation of the structures of sialic acid containing oligosaccharides.
复制标题
高碘酸氧化研究阐明含寡糖唾液酸的结构。
DOI：
10.1016/0006-291x(81)90681-1
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发表时间：
1981
影响因子：
3.1
通讯作者：
Aminoff,D
中科院分区：
文献类型：
--
作者：
Gathmann,WD;Aminoff,D
通过1H-NMR光谱法和甲基化分析确定的唾液酸寡糖糖醇的结构与先前单独从定量高碘酸盐氧化数据得出的结构不一致。在高碘酸盐氧化方法中探索了差异的可能原因。在pH4.5的氧化过程中，高碘酸盐的酸性没有释放游离唾液酸。因此，利用唾液酸残基的阴离子特性分离高碘酸盐氧化产物，从而确定唾液酸在寡糖链中的位置。
The structures of sialo-oligosaccharide alditols as determined by1H-NMR spectrometry together with methylation analysis did not correspond with those derived previously from quantitative periodate oxidation data alone. Possible causes of the discrepancy were explored in the periodate oxidation methodology. No free sialic acid was released by the acidity of the periodate in the course of oxidation at pH 4.5. The anionic properties of the sialic acid residues were therefore utilized to separate the periodate oxidation products and thereby establish the position of the sialic acid in the oligosaccharide chain.